Human T cell leukemia virus type I tax enhances IL-4 gene expression in T cells
The human T cell leukemia virus type-1 (HTLV-1) is the etiologic agent of adult T cell leukemia (ATL). Since the HTLV-I-encoded transactivator Tax has been shown to activate many cellular genes including cytokine genes interleukin (IL-)1α , 2, 5, 6, 8, 10 and 15, we ask whether Tax also affects IL-4...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
24 August 2001
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| In: |
European journal of immunology
Year: 2001, Volume: 31, Issue: 9, Pages: 2623-2632 |
| ISSN: | 1521-4141 |
| DOI: | 10.1002/1521-4141(200109)31:9<2623::AID-IMMU2623>3.0.CO;2-4 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1002/1521-4141(200109)31:9<2623::AID-IMMU2623>3.0.CO;2-4 Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1521-4141%28200109%2931%3A9%3C2623%3A%3AAID-IMMU2623%3E3.0.CO%3B2-4 
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| Author Notes: | Min Li‐Weber, Marco Giaisi, Katerina Chlichlia, Khashayarsha Khazaie and Peter H. Krammer |
| Summary: | The human T cell leukemia virus type-1 (HTLV-1) is the etiologic agent of adult T cell leukemia (ATL). Since the HTLV-I-encoded transactivator Tax has been shown to activate many cellular genes including cytokine genes interleukin (IL-)1α , 2, 5, 6, 8, 10 and 15, we ask whether Tax also affects IL-4 expression. In this study, we show that addition of recombinant Tax proteins greatly enhances IL-4 secretion in human peripheral primary T cells. Transient transfection studies showed that ectopic expression of Tax significantly enhanced IL-4 promoter activity. The IL-4 promoter contains a strong NF-IL6 (PRE-I element) and a NF-AT/NF-κB overlapping site (P1 element). We show that expression of Tax stimulates NF-IL6 binding to the PRE-I element and, consequently, enhancesPRE-I-mediated transcriptional activity. Using Jurkat T cell lines which stably express Tax fused to the hormone binding domain of the human estrogen receptor (ER), we show that Tax enhances endogenous IL-4 mRNA expression and increases IL-4 promoter activity in a hormone-dependent manner. Mutation analysis revealed that the IL-4 PRE-I (NF-IL6 site) and the P1 (NF-AT/NF-κB site) are involved in Tax-mediated transactivation. Our studies provide the first evidence of the functional involvement of Tax in IL-4 gene regulation. |
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| Item Description: | Gesehen am 20.04.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1521-4141 |
| DOI: | 10.1002/1521-4141(200109)31:9<2623::AID-IMMU2623>3.0.CO;2-4 |