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Correlative light microscopy for high-content screening

High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution m...

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Bibliographic Details
Main Authors: Flottmann, Benjamin (Author) , Gunkel, Manuel (Author) , Lisauskas, Tautvydas (Author) , Heilemann, Mike (Author) , Starkuviene-Erfle, Vytaute (Author) , Reymann, Jürgen (Author) , Erfle, Holger (Author)
Format: Article (Journal)
Language:English
Published: November 1, 2013
In: BioTechniques
Year: 2013, Volume: 55, Issue: 5, Pages: 243-252
ISSN:1940-9818
DOI:10.2144/000114099
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.2144/000114099
Verlag, lizenzpflichtig, Volltext: https://www.future-science.com/doi/10.2144/000114099
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Author Notes:Benjamin Flottmann, Manuel Gunkel, Tautvydas Lisauskas, Mike Heilemann, Vytaute Starkuviene, Jürgen Reymann, and Holger Erfle
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Summary:High-throughput microscopy is an effective tool for rapidly collecting data on a large scale. However, high throughput comes at the cost of low spatial resolution. Here we introduce correlative light microscopy by combining fast automated widefield imaging, confocal microscopy and super-resolution microscopy. We demonstrate the potential of this approach for scalable experiments. The workflow consists of a robust approach for selecting cells of interest on a wide-field screening microscope at low resolution and subsequently re-localizing those cells with micrometer precision for confocal and super-resolution imaging. As a case study, we visualized and quantified cis- and trans-Golgi markers at increasing resolution.
Item Description:Gesehen am 26.04.2021
Physical Description:Online Resource
ISSN:1940-9818
DOI:10.2144/000114099

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