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GABAB receptor-mediated inhibition of spontaneous inhibitory synaptic currents in rat midbrain culture.

1. Tight-seal, whole-cell recording was used to study GABAB receptor-mediated inhibition of spontaneous inhibitory synaptic currents in cultured rat midbrain neurones. 2. Spontaneous miniature inhibitory postsynaptic currents (mIPSCs) were recorded in tetrodotoxin (TTX), Cd2+ and Ba2+. (R)-(-)-baclo...

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Main Authors: Rohrbacher, Jutta (Author) , Jarolimek, Wolfgang (Author) , Lewen, Andrea (Author) , Misgeld, Ulrich (Author)
Format: Article (Journal)
Language:English
Published: 01 May 1997
In: The journal of physiology
Year: 1997, Volume: 500, Issue: 3, Pages: 739-749
ISSN:1469-7793
DOI:https://doi.org/10.1113/jphysiol.1997.sp022055
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1113/jphysiol.1997.sp022055
Verlag, lizenzpflichtig, Volltext: https://physoc.onlinelibrary.wiley.com/doi/abs/10.1113/jphysiol.1997.sp022055
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Author Notes:Jutta Rohrbacher, Wolfgang Jarolimek, Andrea Lewen and Ulrich Misgeld
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Summary:1. Tight-seal, whole-cell recording was used to study GABAB receptor-mediated inhibition of spontaneous inhibitory synaptic currents in cultured rat midbrain neurones. 2. Spontaneous miniature inhibitory postsynaptic currents (mIPSCs) were recorded in tetrodotoxin (TTX), Cd2+ and Ba2+. (R)-(-)-baclofen reduced the frequency of mIPSCs through a presynaptic mechanism. The EC50 for this effect was 7 microM. It was antagonized by the GABAB receptor antagonist CGP55845A (0.5 microM). 3. In pertussis toxin (PTX)-treated cultures, some GABAB receptor-mediated reduction of the frequency of mIPSCs persisted. In contrast, PTX treatment totally abolished inhibition of miniature excitatory postsynaptic currents (mEPSCs). 4. In PTX-treated cultures, a saturating concentration of (R)-(-)-baclofen inhibited action potential-generated IPSCs but no EPSCs. 5. PTX treatment abolished the (R)-(-)-baclofen-mediated inhibition of high voltage-activated somatic Ca2+ currents and of spontaneous IPSCs depending on presynaptic Ca2+ entry. 6. We conclude that cellular mechanisms underlying GABAB receptor-mediated inhibition of mIPSCs contribute to auto-inhibition of GABA release.
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Physical Description:Online Resource
ISSN:1469-7793
DOI:https://doi.org/10.1113/jphysiol.1997.sp022055

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