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Preliminary approach to elucidate the role of pigment as a binding site for drugs and chemicals in anagen hair: differential uptake of 3H-haloperidol by pigment-producing compared to non-pigment-producing cell lines

A striking difference was observed for cellular-bound drug in HaCaT and Sk-Mel-1 cells for a fixed drug exposure time of 72 h and varying 3H-haloperidol concentrations in the culture media. Drug uptake was dependent on drug concentration and linearly correlated for both the non-pigment- and the pigm...

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Bibliographic Details
Main Authors: Pötsch-Schneider, Lucia Edeltraud (Author) , Emmerich, Patricia (Author) , Skopp, Gisela (Author)
Format: Article (Journal)
Language:English
Published: February 2002
In: International journal of legal medicine
Year: 2002, Volume: 116, Issue: 1, Pages: 58-61
ISSN:1437-1596
DOI:10.1007/s004140100242
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1007/s004140100242
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Author Notes:L. Pötsch, P. Emmerich, G. Skopp
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Summary:A striking difference was observed for cellular-bound drug in HaCaT and Sk-Mel-1 cells for a fixed drug exposure time of 72 h and varying 3H-haloperidol concentrations in the culture media. Drug uptake was dependent on drug concentration and linearly correlated for both the non-pigment- and the pigment-producing cells which however was different in magnitude. In an additional investigation the time course of drug uptake during 3H-haloperidol exposure (400 pmol/ml; 28 days) revealed increasing drug concentrations in the Sk-Mel-1 population, whereas drug concentrations in the keratinocytes reached a plateau within a short time period. In contrast to the HaCaT cells no tendency to saturation was observed for the pigment-producing cell line. At the end of the experiments 3H-haloperidol concentrations in Sk-Mel-1 were found to be approximately tenfold higher than in HaCaT.
Item Description:Im Titel erscheint die Ziffer 3 hochgestellt
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Physical Description:Online Resource
ISSN:1437-1596
DOI:10.1007/s004140100242

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