Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes
Caspase-8 is a key initiator of death receptor-induced apoptosis. Here we provide evidence that caspase-8 expression is subject to posttranscriptional regulation in human leukocytes. Resting peripheral blood lymphocytes preferentially use a distant splice donor site at the 3′-end of caspase-8 exon 8...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2001
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| In: |
Biochemical and biophysical research communications
Year: 2001, Volume: 289, Issue: 4, Pages: 777-781 |
| ISSN: | 1090-2104 |
| DOI: | 10.1006/bbrc.2001.6055 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/bbrc.2001.6055 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006291X01960553 
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| Author Notes: | Leopold Eckhart, Michael Henry, Antonio M. Santos-Beneit, Ingo Schmitz, Andreas Krueger, Heinz Fischer, Jürgen Bach, Jozef Ban, Sabine Kirchhoff, Peter H. Krammer, Faustino Mollinedo, and Erwin Tschachler |
| Summary: | Caspase-8 is a key initiator of death receptor-induced apoptosis. Here we provide evidence that caspase-8 expression is subject to posttranscriptional regulation in human leukocytes. Resting peripheral blood lymphocytes preferentially use a distant splice donor site at the 3′-end of caspase-8 exon 8 to generate mRNAs with a truncated open reading frame. When lymphocytes were activated, the expression of caspase-8 variants was shifted to caspase-8/a and b which lack the extension of exon 8. The opposite change of the splicing pattern was found in a neutrophil differentiation model. Promyelocytic HL-60 cells mainly expressed caspase-8 mRNAs with the normal exon 8, but the splicing pattern was changed to the distant exon 8 splice site during DMSO-induced differentiation of HL-60 cells. In spite of the presence of these novel mRNAs, the corresponding translation products were not detectable in either cell type. Our findings suggest that leukocyte differentiation and alternative splicing of caspase-8 pre-mRNA are inter-dependent processes. |
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| Item Description: | Elektronische Reproduktion der Druckausgabe Gesehen am 28.04.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1090-2104 |
| DOI: | 10.1006/bbrc.2001.6055 |