Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes
Caspase-8 is a key initiator of death receptor-induced apoptosis. Here we provide evidence that caspase-8 expression is subject to posttranscriptional regulation in human leukocytes. Resting peripheral blood lymphocytes preferentially use a distant splice donor site at the 3′-end of caspase-8 exon 8...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2001
|
| In: |
Biochemical and biophysical research communications
Year: 2001, Jahrgang: 289, Heft: 4, Pages: 777-781 |
| ISSN: | 1090-2104 |
| DOI: | 10.1006/bbrc.2001.6055 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/bbrc.2001.6055 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006291X01960553 
|
| Verfasserangaben: | Leopold Eckhart, Michael Henry, Antonio M. Santos-Beneit, Ingo Schmitz, Andreas Krueger, Heinz Fischer, Jürgen Bach, Jozef Ban, Sabine Kirchhoff, Peter H. Krammer, Faustino Mollinedo, and Erwin Tschachler |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1756127964 | ||
| 003 | DE-627 | ||
| 005 | 20220819184952.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210428s2001 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1006/bbrc.2001.6055 |2 doi | |
| 035 | |a (DE-627)1756127964 | ||
| 035 | |a (DE-599)KXP1756127964 | ||
| 035 | |a (OCoLC)1341407135 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Eckhart, Leopold |d 1971- |e VerfasserIn |0 (DE-588)1232331988 |0 (DE-627)1756128456 |4 aut | |
| 245 | 1 | 0 | |a Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes |c Leopold Eckhart, Michael Henry, Antonio M. Santos-Beneit, Ingo Schmitz, Andreas Krueger, Heinz Fischer, Jürgen Bach, Jozef Ban, Sabine Kirchhoff, Peter H. Krammer, Faustino Mollinedo, and Erwin Tschachler |
| 264 | 1 | |c 2001 | |
| 300 | |a 5 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Elektronische Reproduktion der Druckausgabe | ||
| 500 | |a Gesehen am 28.04.2021 | ||
| 520 | |a Caspase-8 is a key initiator of death receptor-induced apoptosis. Here we provide evidence that caspase-8 expression is subject to posttranscriptional regulation in human leukocytes. Resting peripheral blood lymphocytes preferentially use a distant splice donor site at the 3′-end of caspase-8 exon 8 to generate mRNAs with a truncated open reading frame. When lymphocytes were activated, the expression of caspase-8 variants was shifted to caspase-8/a and b which lack the extension of exon 8. The opposite change of the splicing pattern was found in a neutrophil differentiation model. Promyelocytic HL-60 cells mainly expressed caspase-8 mRNAs with the normal exon 8, but the splicing pattern was changed to the distant exon 8 splice site during DMSO-induced differentiation of HL-60 cells. In spite of the presence of these novel mRNAs, the corresponding translation products were not detectable in either cell type. Our findings suggest that leukocyte differentiation and alternative splicing of caspase-8 pre-mRNA are inter-dependent processes. | ||
| 650 | 4 | |a alternative splicing | |
| 650 | 4 | |a apoptosis | |
| 650 | 4 | |a caspase-8 | |
| 650 | 4 | |a differentiation | |
| 650 | 4 | |a HL-60 | |
| 650 | 4 | |a leukocytes | |
| 650 | 4 | |a neutrophils | |
| 700 | 1 | |a Krammer, Peter H. |d 1946- |e VerfasserIn |0 (DE-588)121766373 |0 (DE-627)081520255 |0 (DE-576)292873417 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biochemical and biophysical research communications |d [Amsterdam] : Elsevier B.V., 1959 |g 289(2001), 4, Seite 777-781 |h Online-Ressource |w (DE-627)254231691 |w (DE-600)1461396-7 |w (DE-576)103373039 |x 1090-2104 |7 nnas |a Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes |
| 773 | 1 | 8 | |g volume:289 |g year:2001 |g number:4 |g pages:777-781 |g extent:5 |a Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes |
| 856 | 4 | 0 | |u https://doi.org/10.1006/bbrc.2001.6055 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.sciencedirect.com/science/article/pii/S0006291X01960553 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210428 | ||
| 993 | |a Article | ||
| 994 | |a 2001 | ||
| 998 | |g 121766373 |a Krammer, Peter H. |m 121766373:Krammer, Peter H. |d 50000 |e 50000PK121766373 |k 0/50000/ |p 10 | ||
| 999 | |a KXP-PPN1756127964 |e 3918640779 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2001","dateIssuedDisp":"2001"}],"id":{"eki":["1756127964"],"doi":["10.1006/bbrc.2001.6055"]},"name":{"displayForm":["Leopold Eckhart, Michael Henry, Antonio M. Santos-Beneit, Ingo Schmitz, Andreas Krueger, Heinz Fischer, Jürgen Bach, Jozef Ban, Sabine Kirchhoff, Peter H. Krammer, Faustino Mollinedo, and Erwin Tschachler"]},"physDesc":[{"extent":"5 S."}],"relHost":[{"origin":[{"dateIssuedDisp":"1959-","dateIssuedKey":"1959","publisher":"Elsevier B.V. ; Academic Press","publisherPlace":"[Amsterdam] ; Orlando, Fla."}],"id":{"issn":["1090-2104"],"zdb":["1461396-7"],"eki":["254231691"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Biochemical and biophysical research communications","title":"Biochemical and biophysical research communications","subtitle":"BBRC"}],"note":["Gesehen am 14.06.2024"],"disp":"Alternative splicing of caspase-8 mRNA during differentiation of human leukocytesBiochemical and biophysical research communications","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"254231691","language":["eng"],"pubHistory":["1.1959 -"],"part":{"volume":"289","text":"289(2001), 4, Seite 777-781","extent":"5","year":"2001","issue":"4","pages":"777-781"},"titleAlt":[{"title":"BBRC"}]}],"title":[{"title":"Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes","title_sort":"Alternative splicing of caspase-8 mRNA during differentiation of human leukocytes"}],"person":[{"role":"aut","display":"Eckhart, Leopold","roleDisplay":"VerfasserIn","given":"Leopold","family":"Eckhart"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Krammer, Peter H.","given":"Peter H.","family":"Krammer"}],"note":["Elektronische Reproduktion der Druckausgabe","Gesehen am 28.04.2021"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"recId":"1756127964"} | ||
| SRT | |a ECKHARTLEOALTERNATIV2001 | ||