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The HPV-16 E5 protein Inhibits TRAIL- and FasL-Mediated apoptosis in human keratinocyte raft cultures

By using raft cultures of the polyclonal HaCaT cell lines stably transfected either with E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as reference, we investigated the effect of the human papillomavirus type 16 (HPV-16) E5 protein on apoptosis. In comparison to conventional monolayer cultures this...

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Main Authors: Kabsch, Kirsten (Author) , Mossadegh, Nina (Author) , Kohl, Annette (Author) , Komposch, Gerda (Author) , Schenkel, Johannes (Author) , Alonso, Angel (Author) , Tomakidi, Pascal (Author)
Format: Article (Journal)
Language:English
Published: March 26, 2004
In: Intervirology
Year: 2004, Volume: 47, Issue: 1, Pages: 48-56
ISSN:1423-0100
DOI:10.1159/000076642
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1159/000076642
Verlag, lizenzpflichtig, Volltext: https://www.karger.com/Article/FullText/76642
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Author Notes:Kirsten Kabsch, Nina Mossadegh, Annette Kohl, Gerda Komposch, Johannes Schenkel, Angel Alonso, Pascal Tomakidi
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Summary:By using raft cultures of the polyclonal HaCaT cell lines stably transfected either with E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as reference, we investigated the effect of the human papillomavirus type 16 (HPV-16) E5 protein on apoptosis. In comparison to conventional monolayer cultures this model system allows analysis of apoptosis under more tissue-like conditions by mimicking the stratified organization of a normal surface epithelium. Apoptosis was triggered either by FasL or TRAIL. Execution of the death program was checked at early and late stages by monitoring procaspase-3 cleavage and DNA fragmentation, respectively. Rafts of E5-expressing keratinocytes were completely protected from apoptosis and showed a background of apoptotic cells as low as the untreated cultures. In contrast, the HaCaT/pMSG cultures revealed a dramatic increase in apoptotic cells upon ligand treatment throughout the epithelial compartment. We conclude that the presence of the HPV-16 E5 protein in our tissue-like model prevents FasL- or TRAIL-mediated apoptosis.
Item Description:Gesehen am 05.05.2021
Physical Description:Online Resource
ISSN:1423-0100
DOI:10.1159/000076642

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