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Aptamer-based proximity labeling guides covalent RNA modification

We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA l...

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Bibliographic Details
Main Authors: Englert, Daniel (Author) , Matveeva, Regina (Author) , Sünbül, Murat (Author) , Wombacher, Richard (Author) , Jäschke, Andres (Author)
Format: Article (Journal)
Language:English
Published: 04 Mar 2021
In: Chemical communications
Year: 2021, Volume: 57, Issue: 28, Pages: 3480-3483
ISSN:1364-548X
DOI:10.1039/D1CC00786F
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/D1CC00786F
Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2021/cc/d1cc00786f
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Author Notes:Daniel Englert, Regina Matveeva, Murat Sunbul, Richard Wombacher and Andres Jäschke
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Summary:We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA labeling in proof-of-principle experiments.
Item Description:Gesehen am 06.05.2021
Physical Description:Online Resource
ISSN:1364-548X
DOI:10.1039/D1CC00786F

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