Aptamer-based proximity labeling guides covalent RNA modification
We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA l...
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| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
04 Mar 2021
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| In: |
Chemical communications
Year: 2021, Jahrgang: 57, Heft: 28, Pages: 3480-3483 |
| ISSN: | 1364-548X |
| DOI: | 10.1039/D1CC00786F |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/D1CC00786F Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2021/cc/d1cc00786f 
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| Verfasserangaben: | Daniel Englert, Regina Matveeva, Murat Sunbul, Richard Wombacher and Andres Jäschke |
| Zusammenfassung: | We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA labeling in proof-of-principle experiments. |
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| Beschreibung: | Gesehen am 06.05.2021 |
| Beschreibung: | Online Resource |
| ISSN: | 1364-548X |
| DOI: | 10.1039/D1CC00786F |