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miR-137 inhibits melanoma cell proliferation through downregulation of GLO1

Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The...

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Main Authors: Lv, Na (Author) , Hao, Shuai (Author) , Luo, Chonglin (Author) , Abukiwan, Alia (Author) , Hao, Ying (Author) , Gai, Fei (Author) , Huang, Weiwei (Author) , Huang, Lingyun (Author) , Xiao, Xueyuan (Author) , Eichmüller, Stefan B. (Author) , He, Dacheng (Author)
Format: Article (Journal)
Language:English
Published: 02 January 2018
In: Science China. Life sciences
Year: 2018, Volume: 61, Issue: 5, Pages: 541-549
ISSN:1869-1889
DOI:10.1007/s11427-017-9138-9
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s11427-017-9138-9
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Author Notes:Na Lv, Shuai Hao, Chonglin Luo, Alia Abukiwan, Ying Hao, Fei Gai, Weiwei Huang, Lingyun Huang, Xueyuan Xiao, Stefan B. Eichmüller, Dacheng He
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Summary:Late-stage melanoma is refractory to current therapies. MicroRNAs (miRNAs) can modulate many physiological and pathological processes of melanoma. Studies have demonstrated that miR-137 acts as a tumor suppressor by inhibiting the proliferation of melanoma cells through targeting multiple mRNAs. The glyoxalase system member glyoxalase 1 (GLO1) is the principal scavenging enzyme of methylglyoxal (MG), a toxic byproduct of glycolysis. Using 35S in vivo/vitro labelling analysis for dynamic proteomics (SiLAD), we found that miR-137 downregulated the expression of GLO1 in melanoma cells. Bioinformatics analysis predicted that GLO1 is a direct target of miR-137. This was validated by dual luciferase reporter assay. Quantitative RT-PCR (qRT-PCR) and western blot analysis indicated that miR-137 could decrease endogenous GLO1 expression. Furthermore, siRNA targeting of GLO1 mimicked inhibition of melanoma cell proliferation caused by miR-137 overexpression. Re-expression of GLO1 was able to restore miR-137-mediated suppression of melanoma cell proliferation. Therefore, these results suggest that miR-137 inhibits the proliferation of melanoma cells by targeting GLO1.
Item Description:Gesehen am 06.05.2021
Physical Description:Online Resource
ISSN:1869-1889
DOI:10.1007/s11427-017-9138-9

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