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Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors

The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated the global economy and claimed more than 1.7 million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designe...

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Autori principali: Hoffmann, Hans-Heinrich (Autore) , Sánchez-Rivera, Francisco J. (Autore) , Schneider, William M. (Autore) , Luna, Joseph M. (Autore) , Soto-Feliciano, Yadira M. (Autore) , Ashbrook, Alison W. (Autore) , Le Pen, Jérémie (Autore) , Leal, Andrew A. (Autore) , Ricardo-Lax, Inna (Autore) , Michailidis, Eleftherios (Autore) , Hao, Yuan (Autore) , Stenzel, Ansgar (Autore) , Peace, Avery (Autore) , Zuber, Johannes (Autore) , Allis, C. David (Autore) , Lowe, Scott W. (Autore) , MacDonald, Margaret R. (Autore) , Poirier, John T. (Autore) , Rice, Charles M. (Autore)
Natura: Article (Journal)
Lingua:inglese
Pubblicazione: 2021
In: Cell host and microbe
Year: 2021, Volume: 29, Fascicolo: 2, Pages: 267-280.e5
ISSN:1934-6069
DOI:10.1016/j.chom.2020.12.009
Accesso online:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.chom.2020.12.009
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1931312820306715
Testo
Note sull'autore:H.-Heinrich Hoffmann, Francisco J. Sánchez-Rivera, William M. Schneider, Joseph M. Luna, Yadira M. Soto-Feliciano, Alison W. Ashbrook, Jérémie Le Pen, Andrew A. Leal, Inna Ricardo-Lax, Eleftherios Michailidis, Yuan Hao, Ansgar F. Stenzel, Avery Peace, Johannes Zuber, C. David Allis, Scott W. Lowe, Margaret R. MacDonald, John T. Poirier, and Charles M. Rice
Descrizione
Riassunto:The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated the global economy and claimed more than 1.7 million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designed a focused high-coverage CRISPR-Cas9 library targeting 332 members of a recently published SARS-CoV-2 protein interactome. We leveraged the compact nature of this library to systematically screen SARS-CoV-2 at two physiologically relevant temperatures along with three related coronaviruses (human coronavirus 229E [HCoV-229E], HCoV-NL63, and HCoV-OC43), allowing us to probe this interactome at a much higher resolution than genome-scale studies. This approach yielded several insights, including potential virus-specific differences in Rab GTPase requirements and glycosylphosphatidylinositol (GPI) anchor biosynthesis, as well as identification of multiple pan-coronavirus factors involved in cholesterol homeostasis. This coronavirus essentiality catalog could inform ongoing drug development efforts aimed at intercepting and treating coronavirus disease 2019 (COVID-19) and help prepare for future coronavirus outbreaks.
Descrizione del documento:Available online 16 December 2020
Gesehen am 10.05.2021
Descrizione fisica:Online Resource
ISSN:1934-6069
DOI:10.1016/j.chom.2020.12.009

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