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Tumor cell-derived angiopoietin-2 promotes metastasis in melanoma

The angiopoietin (Angpt)-TIE signaling pathway controls vascular maturation and maintains the quiescent phenotype of resting vasculature. The contextual agonistic and antagonistic Tie2 ligand ANGPT2 is believed to be exclusively produced by endothelial cells, disrupting constitutive ANGPT1-TIE2 sign...

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Main Authors: Abdul Pari, Ashik Ahmed (Author) , Singhal, Mahak (Author) , Hübers, Corinne (Author) , Mogler, Carolin (Author) , Schieb, Benjamin (Author) , Gampp, Anja (Author) , Gengenbacher, Nicolas (Author) , Reynolds, Louise E. (Author) , Terhardt, Dorothee (Author) , Géraud, Cyrill (Author) , Utikal, Jochen (Author) , Thomas, Markus (Author) , Goerdt, Sergij (Author) , Hodivala-Dilke, Kairbaan M. (Author) , Augustin, Hellmut (Author) , Felcht, Moritz (Author)
Format: Article (Journal)
Language:English
Published: April 17, 2020
In: Cancer research
Year: 2020, Volume: 80, Issue: 12, Pages: 2586-2598
ISSN:1538-7445
DOI:10.1158/0008-5472.CAN-19-2660
Online Access:Verlag, Volltext: https://doi.org/10.1158/0008-5472.CAN-19-2660
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Author Notes:Ashik Ahmed Abdul Pari, Mahak Singhal, Corinne Hübers, Carolin Mogler, Benjamin Schieb, Anja Gampp, Nicolas Gengenbacher, Louise E. Reynolds, Dorothee Terhardt, Cyrill Geraud, Jochen Utikal, Markus Thomas, Sergij Goerdt, Kairbaan M. Hodivala-Dilke, Hellmut G. Augustin, and Moritz Felcht
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Summary:The angiopoietin (Angpt)-TIE signaling pathway controls vascular maturation and maintains the quiescent phenotype of resting vasculature. The contextual agonistic and antagonistic Tie2 ligand ANGPT2 is believed to be exclusively produced by endothelial cells, disrupting constitutive ANGPT1-TIE2 signaling to destabilize the microvasculature during pathologic disorders like inflammation and cancer. However, scattered reports have also portrayed tumor cells as a source of ANGPT2. Employing ISH-based detection of ANGPT2, we found strong tumor cell expression of ANGPT2 in a subset of patients with melanoma. Comparative analysis of biopsies revealed a higher fraction of ANGPT2-expressing tumor cells in metastatic versus primary sites. Tumor cell-expressed Angpt2 was dispensable for primary tumor growth, yet in-depth analysis of primary tumors revealed enhanced intratumoral necrosis upon silencing of tumor cell Angpt2 expression in the absence of significant immune and vascular alterations. Global transcriptional profiling of Angpt2-deficient tumor cells identified perturbations in redox homeostasis and an increased response to cellular oxidative stress. Ultrastructural analyses illustrated a significant increase of dysfunctional mitochondria in Angpt2-silenced tumor cells, thereby resulting in enhanced reactive oxygen species (ROS) production and downstream MAPK stress signaling. Functionally, enhanced ROS in Angpt2-silenced tumor cells reduced colonization potential in vitro and in vivo. Taken together, these findings uncover the hitherto unappreciated role of tumor cell-expressed ANGPT2 as an autocrine-positive regulator of metastatic colonization and validate ANGPT2 as a therapeutic target for a well-defined subset of patients with melanoma. Significance: This study reveals that tumor cells can be a source of ANGPT2 in the tumor microenvironment and that tumor cellderived ANGPT2 augments metastatic colonization by protecting tumor cells from oxidative stress.
Item Description:Gesehen am 10.05.2021
Physical Description:Online Resource
ISSN:1538-7445
DOI:10.1158/0008-5472.CAN-19-2660

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