Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay
For the determination of the HIV protease inhibitors lopinavir and ritonavir in human plasma, plasma ultrafiltrate, and peripheral blood mononuclear cells (PBMCs) a highly sensitive and selective method has been developed, validated, and applied to samples of a healthy volunteer. BD Vacutainer® CPT™...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2007
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| In: |
Journal of chromatography
Year: 2006, Volume: 850, Issue: 1, Pages: 249-258 |
| ISSN: | 1873-376X |
| DOI: | 10.1016/j.jchromb.2006.11.037 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jchromb.2006.11.037 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1570023206009925 
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| Author Notes: | Manuela Ehrhardt, Marion Möck, Walter E. Haefeli, Gerd Mikus, Jürgen Burhenne |
| Summary: | For the determination of the HIV protease inhibitors lopinavir and ritonavir in human plasma, plasma ultrafiltrate, and peripheral blood mononuclear cells (PBMCs) a highly sensitive and selective method has been developed, validated, and applied to samples of a healthy volunteer. BD Vacutainer® CPT™ and Amicon Centriplus® centrifugal filter devices were used for separation of PBMCs and for ultrafiltrate generation, respectively. After liquid/liquid-extraction extracts were chromatographed isocratically within 6min on a Jupiter Proteo column. The drugs were quantified using 2H5-saquinavir as internal standard and electrospray tandem mass spectrometry in the selected reaction monitoring mode. Limits of quantification for both analytes were 4.0ng/mL in plasma, 0.2ng/mL in ultrafiltrate, and 0.1ng/cell pellet (∼3×106 cells) in PBMCs. The calibration ranges were linear over more than three logs with an over-all accuracy varying between 98.7% and 111.5% and an over-all precision ranging from 6.2% to 14.0% (SD batch-to-batch). After a regular oral dose of Kaletra® (400mg lopinavir, 100mg ritonavir) analyte concentrations were detectable over a full dosing interval in plasma, ultrafiltrate, and PBMCs. The method is well suited for monitoring of free and total plasma, and intracellular lopinavir/ritonavir concentrations in samples from clinical trials. |
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| Item Description: | Online 11 December 2006 Gesehen am 12.05.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1873-376X |
| DOI: | 10.1016/j.jchromb.2006.11.037 |