Cover Image

Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay

For the determination of the HIV protease inhibitors lopinavir and ritonavir in human plasma, plasma ultrafiltrate, and peripheral blood mononuclear cells (PBMCs) a highly sensitive and selective method has been developed, validated, and applied to samples of a healthy volunteer. BD Vacutainer® CPT™...

Full description

Saved in:
Bibliographic Details
Main Authors: Ehrhardt, Manuela (Author) , Möck, Marion (Author) , Haefeli, Walter E. (Author) , Mikus, Gerd (Author) , Burhenne, Jürgen (Author)
Format: Article (Journal)
Language:English
Published: 2007
In: Journal of chromatography
Year: 2006, Volume: 850, Issue: 1, Pages: 249-258
ISSN:1873-376X
DOI:10.1016/j.jchromb.2006.11.037
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jchromb.2006.11.037
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1570023206009925
Get full text
Author Notes:Manuela Ehrhardt, Marion Möck, Walter E. Haefeli, Gerd Mikus, Jürgen Burhenne

MARC

LEADER 00000caa a2200000 c 4500
001 1757720391
003 DE-627
005 20230428185534.0
007 cr uuu---uuuuu
008 210512r20072006xx |||||o 00| ||eng c
024 7 |a 10.1016/j.jchromb.2006.11.037  |2 doi 
035 |a (DE-627)1757720391 
035 |a (DE-599)KXP1757720391 
035 |a (OCoLC)1341409073 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Ehrhardt, Manuela  |d 1976-  |e VerfasserIn  |0 (DE-588)133265897  |0 (DE-627)539648221  |0 (DE-576)299736210  |4 aut 
245 1 0 |a Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay  |c Manuela Ehrhardt, Marion Möck, Walter E. Haefeli, Gerd Mikus, Jürgen Burhenne 
264 1 |c 2007 
300 |a 10 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Online 11 December 2006 
500 |a Gesehen am 12.05.2021 
520 |a For the determination of the HIV protease inhibitors lopinavir and ritonavir in human plasma, plasma ultrafiltrate, and peripheral blood mononuclear cells (PBMCs) a highly sensitive and selective method has been developed, validated, and applied to samples of a healthy volunteer. BD Vacutainer® CPT™ and Amicon Centriplus® centrifugal filter devices were used for separation of PBMCs and for ultrafiltrate generation, respectively. After liquid/liquid-extraction extracts were chromatographed isocratically within 6min on a Jupiter Proteo column. The drugs were quantified using 2H5-saquinavir as internal standard and electrospray tandem mass spectrometry in the selected reaction monitoring mode. Limits of quantification for both analytes were 4.0ng/mL in plasma, 0.2ng/mL in ultrafiltrate, and 0.1ng/cell pellet (∼3×106 cells) in PBMCs. The calibration ranges were linear over more than three logs with an over-all accuracy varying between 98.7% and 111.5% and an over-all precision ranging from 6.2% to 14.0% (SD batch-to-batch). After a regular oral dose of Kaletra® (400mg lopinavir, 100mg ritonavir) analyte concentrations were detectable over a full dosing interval in plasma, ultrafiltrate, and PBMCs. The method is well suited for monitoring of free and total plasma, and intracellular lopinavir/ritonavir concentrations in samples from clinical trials. 
534 |c 2006 
650 4 |a Kaletra 
650 4 |a LC/MS/MS 
650 4 |a Lopinavir 
650 4 |a PBMC 
650 4 |a Protein binding 
650 4 |a Ritonavir 
700 1 |a Möck, Marion  |d 1982-  |e VerfasserIn  |0 (DE-588)136108490  |0 (DE-627)694086428  |0 (DE-576)300836317  |4 aut 
700 1 |a Haefeli, Walter E.  |d 1958-  |e VerfasserIn  |0 (DE-588)124572359  |0 (DE-627)656806141  |0 (DE-576)340514221  |4 aut 
700 1 |a Mikus, Gerd  |e VerfasserIn  |0 (DE-588)110903137  |0 (DE-627)691097941  |0 (DE-576)336988710  |4 aut 
700 1 |a Burhenne, Jürgen  |d 1963-  |e VerfasserIn  |0 (DE-588)1034228889  |0 (DE-627)745126987  |0 (DE-576)381858197  |4 aut 
773 0 8 |i Enthalten in  |t Journal of chromatography  |d New York, NY [u.a.] : Science Direct, 2002  |g 850(2007), 1, Seite 249-258  |h Online-Ressource  |w (DE-627)302467505  |w (DE-600)1491259-4  |w (DE-576)094950261  |x 1873-376X  |7 nnas  |a Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay 
773 1 8 |g volume:850  |g year:2007  |g number:1  |g pages:249-258  |g extent:10  |a Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay 
856 4 0 |u https://doi.org/10.1016/j.jchromb.2006.11.037  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.sciencedirect.com/science/article/pii/S1570023206009925  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20210512 
993 |a Article 
994 |a 2007 
998 |g 1034228889  |a Burhenne, Jürgen  |m 1034228889:Burhenne, Jürgen  |d 50000  |e 50000PB1034228889  |k 0/50000/  |p 5  |y j 
998 |g 110903137  |a Mikus, Gerd  |m 110903137:Mikus, Gerd  |d 910000  |d 910100  |d 50000  |e 910000PM110903137  |e 910100PM110903137  |e 50000PM110903137  |k 0/910000/  |k 1/910000/910100/  |k 0/50000/  |p 4 
998 |g 124572359  |a Haefeli, Walter E.  |m 124572359:Haefeli, Walter E.  |d 910000  |d 910100  |e 910000PH124572359  |e 910100PH124572359  |k 0/910000/  |k 1/910000/910100/  |p 3 
999 |a KXP-PPN1757720391  |e 3927650013 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay","title":"Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay"}],"person":[{"family":"Ehrhardt","given":"Manuela","roleDisplay":"VerfasserIn","role":"aut","display":"Ehrhardt, Manuela"},{"display":"Möck, Marion","given":"Marion","roleDisplay":"VerfasserIn","role":"aut","family":"Möck"},{"family":"Haefeli","role":"aut","roleDisplay":"VerfasserIn","given":"Walter E.","display":"Haefeli, Walter E."},{"family":"Mikus","display":"Mikus, Gerd","given":"Gerd","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Burhenne","roleDisplay":"VerfasserIn","role":"aut","given":"Jürgen","display":"Burhenne, Jürgen"}],"language":["eng"],"name":{"displayForm":["Manuela Ehrhardt, Marion Möck, Walter E. Haefeli, Gerd Mikus, Jürgen Burhenne"]},"id":{"eki":["1757720391"],"doi":["10.1016/j.jchromb.2006.11.037"]},"physDesc":[{"extent":"10 S."}],"origin":[{"dateIssuedDisp":"2007","dateIssuedKey":"2007"}],"relHost":[{"language":["eng"],"disp":"Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assayJournal of chromatography","physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisher":"Science Direct","dateIssuedDisp":"2002-","dateIssuedKey":"2002","publisherPlace":"New York, NY [u.a.]"}],"title":[{"title":"Journal of chromatography","title_sort":"Journal of chromatography"}],"titleAlt":[{"title":"Journal of chromatography / B"}],"id":{"issn":["1873-376X"],"eki":["302467505"],"zdb":["1491259-4"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"pubHistory":["766.2002 - 879.2011; Vol. 880.2012 -"],"part":{"volume":"850","issue":"1","text":"850(2007), 1, Seite 249-258","extent":"10","year":"2007","pages":"249-258"},"recId":"302467505","note":["Gesehen am 10.06.2020"]}],"note":["Online 11 December 2006","Gesehen am 12.05.2021"],"recId":"1757720391","type":{"bibl":"article-journal","media":"Online-Ressource"}} 
SRT |a EHRHARDTMAMONITORING2007 

Similar Items