Transforming growth factor β (TGF-β) levels in the conditioned media of human bone cells: relationship to donor age, bone volume, and concentration of TGF-β in human bone matrix in vivo
Transforming growth factor-β (TGF-β) is thought to play an important role in human bone remodeling. In the present study, we examined constitutive differences in TGF-β levels in primary bone cell cultures from the iliac crest of 112 women, aged 28-79 years. TGF-β1 was the major TGF-β isoform in the...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
24 May 1999
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| In: |
Bone
Year: 1999, Volume: 24, Issue: 6, Pages: 565-569 |
| ISSN: | 1873-2763 |
| DOI: | 10.1016/S8756-3282(99)00082-4 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/S8756-3282(99)00082-4 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S8756328299000824 
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| Author Notes: | H. Bismar, T. Klöppinger, E.M. Schuster, S. Balbach, I. Diel, R. Ziegler, and J. Pfeilschifter |
| Summary: | Transforming growth factor-β (TGF-β) is thought to play an important role in human bone remodeling. In the present study, we examined constitutive differences in TGF-β levels in primary bone cell cultures from the iliac crest of 112 women, aged 28-79 years. TGF-β1 was the major TGF-β isoform in the conditioned media, as determined by neutralizing TGF-β activity with specific antibodies against TGF-β1-3 in the mink lung cell bioassay, and by enzyme-linked immunoassay (ELISA). TGF-β1 levels in the conditioned media did not change with donor age. There was a lack of association between TGF-β levels in vitro and the concentration of matrix-associated TGF-β in vivo. TGF-β1 levels failed to be associated with the local trabecular bone volume in the complete study population (r = +0.15, p = 0.16, n = 89). A significant association between TGF-β1 levels and bone volume was present in premenopausal women (r = +0.39, p = 0.02, n = 33), but was largely accounted for by the two samples with the highest TGF-β concentrations. In conclusion, our data suggest that TGF-β1 is the major TGF-β isoform produced by human bone cells in vitro, and that the constitutive secretion of TGF-β by bone cells does not change with age. Whether constitutive differences in TGF-β secretion may be a determinant of human bone mass remains to be clarified in further studies. |
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| Item Description: | Gesehen am 14.05.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1873-2763 |
| DOI: | 10.1016/S8756-3282(99)00082-4 |