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Transforming growth factor β (TGF-β) levels in the conditioned media of human bone cells: relationship to donor age, bone volume, and concentration of TGF-β in human bone matrix in vivo

Transforming growth factor-β (TGF-β) is thought to play an important role in human bone remodeling. In the present study, we examined constitutive differences in TGF-β levels in primary bone cell cultures from the iliac crest of 112 women, aged 28-79 years. TGF-β1 was the major TGF-β isoform in the...

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Hauptverfasser: Bismar, Hanadi (VerfasserIn) , Klöppinger, T (VerfasserIn) , Schuster, Elke Maria (VerfasserIn) , Balbach, S (VerfasserIn) , Diel, Ingo J. (VerfasserIn) , Ziegler, Reinhard (VerfasserIn) , Pfeilschifter, Johannes (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 24 May 1999
In: Bone
Year: 1999, Jahrgang: 24, Heft: 6, Pages: 565-569
ISSN:1873-2763
DOI:10.1016/S8756-3282(99)00082-4
Online-Zugang:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/S8756-3282(99)00082-4
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S8756328299000824
Volltext
Verfasserangaben:H. Bismar, T. Klöppinger, E.M. Schuster, S. Balbach, I. Diel, R. Ziegler, and J. Pfeilschifter
Beschreibung
Zusammenfassung:Transforming growth factor-β (TGF-β) is thought to play an important role in human bone remodeling. In the present study, we examined constitutive differences in TGF-β levels in primary bone cell cultures from the iliac crest of 112 women, aged 28-79 years. TGF-β1 was the major TGF-β isoform in the conditioned media, as determined by neutralizing TGF-β activity with specific antibodies against TGF-β1-3 in the mink lung cell bioassay, and by enzyme-linked immunoassay (ELISA). TGF-β1 levels in the conditioned media did not change with donor age. There was a lack of association between TGF-β levels in vitro and the concentration of matrix-associated TGF-β in vivo. TGF-β1 levels failed to be associated with the local trabecular bone volume in the complete study population (r = +0.15, p = 0.16, n = 89). A significant association between TGF-β1 levels and bone volume was present in premenopausal women (r = +0.39, p = 0.02, n = 33), but was largely accounted for by the two samples with the highest TGF-β concentrations. In conclusion, our data suggest that TGF-β1 is the major TGF-β isoform produced by human bone cells in vitro, and that the constitutive secretion of TGF-β by bone cells does not change with age. Whether constitutive differences in TGF-β secretion may be a determinant of human bone mass remains to be clarified in further studies.
Beschreibung:Gesehen am 14.05.2021
Beschreibung:Online Resource
ISSN:1873-2763
DOI:10.1016/S8756-3282(99)00082-4

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