Inadequate binding of immune regulator factor H is associated with sensitivity of Borrelia lusitaniae to human complement
Spirochetes belonging to the Borrelia burgdorferi sensu lato complex differ in resistance to complement-mediated killing by human serum. Here, we characterize complement sensitivity of a panel of B. lusitaniae isolates derived from ticks collected in Germany and Portugal as well as one patient-deriv...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
7 September 2010
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| In: |
Infection and immunity
Year: 2010, Jahrgang: 78, Heft: 11, Pages: 4467-4476 |
| ISSN: | 1098-5522 |
| DOI: | 10.1128/IAI.00138-10 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/10.1128/IAI.00138-10 Verlag, lizenzpflichtig, Volltext: https://iai.asm.org/content/78/11/4467 
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| Verfasserangaben: | Roswitha Dieterich, Claudia Hammerschmidt, Dania Richter, Christine Skerka, Reinhard Wallich, Franz-Rainer Matuschka, Peter F. Zipfel, and Peter Kraiczy |
| Zusammenfassung: | Spirochetes belonging to the Borrelia burgdorferi sensu lato complex differ in resistance to complement-mediated killing by human serum. Here, we characterize complement sensitivity of a panel of B. lusitaniae isolates derived from ticks collected in Germany and Portugal as well as one patient-derived isolate, PoHL. All isolates are highly susceptible to complement-mediated lysis in human serum and activate complement predominantly by the alternative pathway, leading to an increased deposition of complement components C3, C6, and the terminal complement complex. Interestingly, serum-sensitive B. lusitaniae isolates were able to bind immune regulator factor H (CFH), and some strains also bound CFH-related protein 1 (CFHR1) and CFHR2. Moreover, CFH bound to the surface of B. lusitaniae was inefficient in mediating C3b conversion. Furthermore, the identification and characterization of a potential CFH-binding protein, OspE, revealed that this molecule possesses a significantly reduced binding capacity for CFH compared to that of CFH-binding OspE paralogs expressed by various serum-resistant Borrelia species. This finding suggests that a reduced binding capability of CFH is associated with an increased serum sensitivity of B. lusitaniae to human complement. |
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| Beschreibung: | Gesehen am 17.05.2021 |
| Beschreibung: | Online Resource |
| ISSN: | 1098-5522 |
| DOI: | 10.1128/IAI.00138-10 |