Rapid and reliable genotyping of the C181T polymorphism in the bradykinin B2 receptor Gene
Bradykinin is not only a mediator of pain and inflammation but also a potent vasodilator and is likely to contribute to the cardioprotective effects of angiotensin-converting enzyme inhibitors. The B 2 receptor (B 2 -R) mediating most of the inflammatory and cardiovascular effects of bradykinin repr...
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| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2003
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| In: |
Clinical chemistry and laboratory medicine
Year: 2003, Jahrgang: 41, Heft: 3, Pages: 281-283 |
| ISSN: | 1437-4331 |
| DOI: | 10.1515/CCLM.2003.044 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1515/CCLM.2003.044 Verlag, lizenzpflichtig, Volltext: https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2003.044/html 
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| Verfasserangaben: | Johanna Weiss, Christiane Hesse and Walter Emil Haefeli |
| Zusammenfassung: | Bradykinin is not only a mediator of pain and inflammation but also a potent vasodilator and is likely to contribute to the cardioprotective effects of angiotensin-converting enzyme inhibitors. The B 2 receptor (B 2 -R) mediating most of the inflammatory and cardiovascular effects of bradykinin represents a candidate gene likely involved in the complex genetic causes of common chronic disorders such as hypertension. The C 181 T polymorphism of the B 2 -R gene influences the potency of bradykinin and there is evidence of a possible role of this polymorphism in the development of hypertension and end-stage renal disease. We developed and validated a new, rapid, and reliable method for the detection of the C 181 T polymorphism based on the hybridization probes format on the LightCycler™, which is superior to the conventional PCR-RFLP method commonly used to detect this polymorphism. It allows the solid evaluation of large patient populations with the best time. |
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| Beschreibung: | Gesehen am 25.05.2021 |
| Beschreibung: | Online Resource |
| ISSN: | 1437-4331 |
| DOI: | 10.1515/CCLM.2003.044 |