An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation
In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5′-UTR, introns, 3′-UTR and terminator elements. Our data revealed that, in contrast to a...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1 February 2013
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| In: |
FEBS letters
Year: 2013, Volume: 587, Issue: 6, Pages: 706-710 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2013.01.045 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.febslet.2013.01.045 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0014579313000902 
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| Author Notes: | Elena Dadami, Mirko Moser, Michele Zwiebel, Gabi Krczal, Michael Wassenegger, Athanasios Dalakouras |
| Summary: | In plants, transgenes are generally more sensitive against RNA silencing than endogenes are. In this study, we generated a transgene that structurally mimicks an endogene. It is composed of endogenous promoter, 5′-UTR, introns, 3′-UTR and terminator elements. Our data revealed that, in contrast to a conventional transgene, an endogene-resembling transgene was more stably expressed and poorly processed into small RNAs. In addition, although both constructs triggered methylation of homologous DNA sequences at similar levels, the endogene-resembling transgene exhibited significantly delayed onset of local and systemic silencing. |
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| Item Description: | Gesehen am 01.06.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2013.01.045 |