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Heterozygous Tbk1 loss has opposing effects in early and late stages of ALS in mice

Heterozygous loss-of-function mutations of TANK-binding kinase 1 (TBK1) cause familial ALS, yet downstream mechanisms of TBK1 mutations remained elusive. TBK1 is a pleiotropic kinase involved in the regulation of selective autophagy and inflammation. We show that heterozygous Tbk1 deletion alone doe...

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Main Authors: Brenner, David (Author) , Sieverding, Kirsten (Author) , Kröger, Clara (Author) , Lüningschrör, Patrick (Author) , Buck, Eva (Author) , Mungwa, Simon (Author) , Fischer, Lena (Author) , Brockmann, Sarah J. (Author) , Ulmer, Johannes (Author) , Bliederhäuser, Corinna (Author) , Philibert, Clémentine E. (Author) , Satoh, Takashi (Author) , Akira, Shizuo (Author) , Boillée, Séverine (Author) , Mayer, Benjamin (Author) , Sendtner, Michael (Author) , Ludolph, Albert C. (Author) , Danzer, Karin M. (Author) , Lobsiger, Christian S. (Author) , Freischmidt, Axel (Author) , Weishaupt, Jochen H. (Author)
Format: Article (Journal)
Language:English
Published: January 11, 2019
In: Journal of experimental medicine
Year: 2019, Volume: 216, Issue: 2, Pages: 267-278
ISSN:1540-9538
DOI:10.1084/jem.20180729
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1084/jem.20180729
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Author Notes:David Brenner, Kirsten Sieverding, Clara Bruno, Patrick Lüningschrör, Eva Buck, Simon Mungwa, Lena Fischer, Sarah J. Brockmann, Johannes Ulmer, Corinna Bliederhäuser, Clémentine E. Philibert, Takashi Satoh, Shizuo Akira, Séverine Boillée, Benjamin Mayer, Michael Sendtner, Albert C. Ludolph, Karin M. Danzer, Christian S. Lobsiger, Axel Freischmidt, and Jochen H. Weishaupt
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Summary:Heterozygous loss-of-function mutations of TANK-binding kinase 1 (TBK1) cause familial ALS, yet downstream mechanisms of TBK1 mutations remained elusive. TBK1 is a pleiotropic kinase involved in the regulation of selective autophagy and inflammation. We show that heterozygous Tbk1 deletion alone does not lead to signs of motoneuron degeneration or disturbed autophagy in mice during a 200-d observation period. Surprisingly, however, hemizygous deletion of Tbk1 inversely modulates early and late disease phases in mice additionally overexpressing ALS-linked SOD1G93A, which represents a “second hit” that induces both neuroinflammation and proteostatic dysregulation. At the early stage, heterozygous Tbk1 deletion impairs autophagy in motoneurons and prepones both the clinical onset and muscular denervation in SOD1G93A/Tbk1+/− mice. At the late disease stage, however, it significantly alleviates microglial neuroinflammation, decelerates disease progression, and extends survival. Our results indicate a profound effect of TBK1 on brain inflammatory cells under pro-inflammatory conditions and point to a complex, two-edged role of TBK1 in SOD1-linked ALS.
Item Description:Gesehen am 16.06.2021
Physical Description:Online Resource
ISSN:1540-9538
DOI:10.1084/jem.20180729

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