Cover Image

Establishment of a highly specific detection system for GB virus C (GBV-C) minus-strand RNA

Although the clinical relevance of GB virus C (GBV-C) is still elusive, this virus has been found with high prevalence in several groups of patients with liver disease. As was shown for hepatitis C virus (HCV), minus-strand RNA is supposed to function as a replicative intermediate. We have establish...

Full description

Saved in:
Bibliographic Details
Main Authors: Seipp, Stefanie (Author) , Goeser, Tobias (Author) , Theilmann, Lorenz (Author) , Kallinowski, Birgit (Author)
Format: Article (Journal)
Language:English
Published: 18 September 1998
In: Virus research
Year: 1998, Volume: 56, Issue: 2, Pages: 183-189
ISSN:1872-7492
DOI:10.1016/S0168-1702(98)00071-9
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0168-1702(98)00071-9
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0168170298000719
Get full text
Author Notes:Stefanie Seipp, Tobias Goeser, Lorenz Theilmann, Birgit Kallinowski
Description
Summary:Although the clinical relevance of GB virus C (GBV-C) is still elusive, this virus has been found with high prevalence in several groups of patients with liver disease. As was shown for hepatitis C virus (HCV), minus-strand RNA is supposed to function as a replicative intermediate. We have established a reliable and sensitive detection system for GBV-C minus-strand RNA based on nested RT-PCR (reverse transcription-polymerase chain reaction) with a tagged primer system. Sensitivity and specificity was extensively tested using in-vitro transcribed GBV-C sequences and genomic viral RNA. Specificity of the amplified fragments was proven by Southern blot hybridization. Using this detection system, we found the presence of GBV-C minus-strand RNA in 6/41 (14.6%) sera of GBV-C infected or GBV-C/HCV coinfected patients. No correlation with virological parameters such as amount of GBV-C plus-strand RNA, genotype or titer of HCV could be detected.
Item Description:Gesehen am 24.06.2021
Physical Description:Online Resource
ISSN:1872-7492
DOI:10.1016/S0168-1702(98)00071-9

Similar Items