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Host defence against Staphylococcus aureus biofilms by polymorphonuclear neutrophils: oxygen radical production but not phagocytosis depends on opsonisation with immunoglobulin G

Bacterial biofilms are increasingly recognised as a major cause of persistent infection and destructive inflammatory processes. In patients with biofilm infection, massive infiltration of leukocytes, particularly polymorphonuclear neutrophils is seen, and previous in vitro studies showed that PMN we...

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Bibliographische Detailangaben
Hauptverfasser: Stroh, Petra (VerfasserIn) , Günther, Frank (VerfasserIn) , Meyle, Eva (VerfasserIn) , Prior, Birgit (VerfasserIn) , Wagner, Christof (VerfasserIn) , Hänsch, Gertrud Maria (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2011
In: Immunobiology
Year: 2011, Jahrgang: 216, Heft: 3, Pages: 351-357
ISSN:1878-3279
DOI:10.1016/j.imbio.2010.07.009
Online-Zugang:Verlag, lizenzpflichtig, Volltext: http://dx.doi.org/10.1016/j.imbio.2010.07.009
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0171298510001439
Volltext
Verfasserangaben:Petra Stroh, Frank Günther, Eva Meyle, Birgit Prior, Christof Wagner, Gertrud Maria Hänsch
Beschreibung
Zusammenfassung:Bacterial biofilms are increasingly recognised as a major cause of persistent infection and destructive inflammatory processes. In patients with biofilm infection, massive infiltration of leukocytes, particularly polymorphonuclear neutrophils is seen, and previous in vitro studies showed that PMN were able to phagocytose Staphylococcus aureus biofilms. We now addressed the question whether opsonisation of biofilms with immunoglobulin G and complement enhances the efficiency of phagocytosis, as it has been shown for "free-living" planktonic bacteria and other particulate materials. We found that incubation of biofilms with normal human serum resulted in IgG binding and in complement activation with deposits on the biofilm of C3bi. This "opsonisation", however, did not affect the adherence of PMN to the biofilms nor did it enhance degranulation or phagocytosis. The clearance of biofilms, however, was increased, and the oxygen radical production by the PMN depended critically on the coating of biofilms with IgG.
Beschreibung:Gesehen am 30.06.2021
Beschreibung:Online Resource
ISSN:1878-3279
DOI:10.1016/j.imbio.2010.07.009

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