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Structural characterization of the principal mRNA-export factor Mex67-Mtr2 from Chaetomium thermophilum

Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, es...

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Bibliographic Details
Main Authors: Aibara, Shintaro (Author) , Dimitrova-Paternoga, Lyudmila Nikolova (Author) , Hurt, Ed (Author)
Format: Article (Journal)
Language:English
Published: 27 June 2015
In: Acta crystallographica. Section F, Structural biology communications
Year: 2015, Volume: 71, Issue: 7, Pages: 876-888
ISSN:2053-230X
DOI:10.1107/S2053230X15008766
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1107/S2053230X15008766
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Author Notes:Shintaro Aibara, Eugene Valkov, Meindert H. Lamers, Lyudmila Dimitrova, Ed Hurt and Murray Stewart
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Summary:Members of the Mex67–Mtr2/NXF–NXT1 family are the principal mediators of the nuclear export of mRNA. Mex67/NXF1 has a modular structure based on four domains (RRM, LRR, NTF2-like and UBA) that are thought to be present across species, although the level of sequence conservation between organisms, especially in lower eukaryotes, is low. Here, the crystal structures of these domains from the thermophilic fungus Chaetomium thermophilum are presented together with small-angle X-ray scattering (SAXS) and in vitro RNA-binding data that indicate that, not withstanding the limited sequence conservation between different NXF family members, the molecules retain similar structural and RNA-binding properties. Moreover, the resolution of crystal structures obtained with the C. thermophilum domains was often higher than that obtained previously and, when combined with solution and biochemical studies, provided insight into the structural organization, self-association and RNA-binding properties of Mex67–Mtr2 that facilitate mRNA nuclear export.
Item Description:Gesehen am 14.07.2021
Physical Description:Online Resource
ISSN:2053-230X
DOI:10.1107/S2053230X15008766

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