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Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels-Alder bioconjugation

Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels-Ald...

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Bibliographic Details
Main Authors: Schoch, Juliane (Author) , Jäschke, Andres (Author)
Format: Article (Journal)
Language:English
Published: 28 Jan 2013
In: RSC Advances
Year: 2013, Volume: 3, Issue: 13, Pages: 4181-4183
ISSN:2046-2069
DOI:10.1039/C3RA40373D
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1039/C3RA40373D
Verlag, lizenzpflichtig, Volltext: https://pubs.rsc.org/en/content/articlelanding/2013/ra/c3ra40373d
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Author Notes:Juliane Schoch and Andres Jäschke
Description
Summary:Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels-Alder reaction.
Item Description:Gesehen am 15.07.2021
Physical Description:Online Resource
ISSN:2046-2069
DOI:10.1039/C3RA40373D

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