Cover Image

Single-cell transcriptomics reveals immune response of intestinal cell types to viral infection

Human intestinal epithelial cells form a primary barrier protecting us from pathogens, yet only limited knowledge is available about individual contribution of each cell type to mounting an immune response against infection. Here, we developed a framework combining single-cell RNA-Seq and highly mul...

Full description

Saved in:
Bibliographic Details
Main Authors: Triana, Sergio (Author) , Stanifer, Megan (Author) , Metz Zumarán, Camila (Author) , Shahraz, Mohammed (Author) , Mukenhirn, Markus (Author) , Kee, Carmon (Author) , Serger, Clara (Author) , Koschny, Ronald (Author) , Ordonez-Rueda, Diana (Author) , Paulsen, Malte (Author) , Benes, Vladimir (Author) , Boulant, Steeve (Author) , Alexandrov, Theodore (Author)
Format: Article (Journal)
Language:English
Published: 26 July 2021
In: Molecular systems biology
Year: 2021, Volume: 17, Issue: 7, Pages: 1-16
ISSN:1744-4292
DOI:10.15252/msb.20209833
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.15252/msb.20209833
Verlag, lizenzpflichtig, Volltext: https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=DynamicDOIArticle&SrcApp=WOS&KeyAID=10.15252%2Fmsb.20209833&DestApp=DOI&SrcAppSID=C1FoMGRrimTkii16l55&SrcJTitle=MOLECULAR+SYSTEMS+BIOLOGY&DestDOIRegistrantName=EMBO
Get full text
Author Notes:Sergio Triana, Megan L. Stanifer, Camila Metz-Zumaran, Mohammed Shahraz, Markus Mukenhirn, Carmon Kee, Clara Serger, Ronald Koschny, Diana Ordonez-Rueda, Malte Paulsen, Vladimir Benes, Steeve Boulant & Theodore Alexandrov
Description
Summary:Human intestinal epithelial cells form a primary barrier protecting us from pathogens, yet only limited knowledge is available about individual contribution of each cell type to mounting an immune response against infection. Here, we developed a framework combining single-cell RNA-Seq and highly multiplex RNA FISH and applied it to human intestinal organoids infected with human astrovirus, a model human enteric virus. We found that interferon controls the infection and that astrovirus infects all major cell types and lineages and induces expression of the cell proliferation marker MKI67. Intriguingly, each intestinal epithelial cell lineage exhibits a unique basal expression of interferon-stimulated genes and, upon astrovirus infection, undergoes an antiviral transcriptional reprogramming by upregulating distinct sets of interferon-stimulated genes. These findings suggest that in the human intestinal epithelium, each cell lineage plays a unique role in resolving virus infection. Our framework is applicable to other organoids and viruses, opening new avenues to unravel roles of individual cell types in viral pathogenesis.
Item Description:Gesehen am 06.10.2021
Physical Description:Online Resource
ISSN:1744-4292
DOI:10.15252/msb.20209833

Similar Items