A defined range of guard cell calcium oscillation parameters encodes stomatal movements
Oscillations in cytosolic calcium concentration ([Ca2+]cyt) are central regulators of signal transduction cascades1, although the roles of individual [Ca2+]cyt oscillation parameters in regulating downstream physiological responses remain largely unknown. In plants, guard cells integrate environment...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
28 June 2001
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| In: |
Nature
Year: 2001, Volume: 411, Issue: 6841, Pages: 1053-1057 |
| ISSN: | 1476-4687 |
| DOI: | 10.1038/35082575 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/35082575 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/35082575 
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| Author Notes: | Gethyn J. Allen, Sarah P. Chu, Carrie L. Harrington, Karin Schumacher, Thomas Hoffmann, Yat Y. Tang, Erwin Grill & Julian I. Schroeder |
| Summary: | Oscillations in cytosolic calcium concentration ([Ca2+]cyt) are central regulators of signal transduction cascades1, although the roles of individual [Ca2+]cyt oscillation parameters in regulating downstream physiological responses remain largely unknown. In plants, guard cells integrate environmental and endogenous signals to regulate the aperture of stomatal pores2 and [Ca2+]cyt oscillations are a fundamental component of stomatal closure3,4. Here we systematically vary [Ca2+]cyt oscillation parameters in Arabidopsis guard cells using a ‘calcium clamp’3,5,6,7 and show that [Ca2+]cyt controls stomatal closure by two mechanisms. Short-term ‘calcium-reactive’ closure occurred rapidly when [Ca2+]cyt was elevated, whereas the degree of long-term steady-state closure was ‘calcium programmed’ by [Ca2+]cyt oscillations within a defined range of frequency, transient number, duration and amplitude. Furthermore, in guard cells of the gca2 mutant8, [Ca2+]cyt oscillations induced by abscisic acid and extracellular calcium had increased frequencies and reduced transient duration, and steady-state stomatal closure was abolished. Experimentally imposing [Ca2+]cyt oscillations with parameters that elicited closure in the wild type restored long-term closure in gca2 stomata. These data show that a defined window of guard cell [Ca2+]cyt oscillation parameters programs changes in steady-state stomatal aperture. |
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| Item Description: | Gesehen am 07.10.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1476-4687 |
| DOI: | 10.1038/35082575 |