Identification of an Arabidopsis mitochondrial succinate-fumarate translocator
Complementation of a yeast acr1 mutant carrying a deletion of the succinate/fumarate carrier gene enabled functional identification of a mitochondrial succinate translocator from Arabidopsis thaliana (AtmSFC1). Thus complementation of yeast mutants is applicable also for identification and character...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 16, 2003
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| In: |
FEBS letters
Year: 2003, Volume: 534, Issue: 1/3, Pages: 87-92 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/S0014-5793(02)03782-1 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0014-5793(02)03782-1 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/S0014-5793%2802%2903782-1 
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| Author Notes: | Elisabetta Catoni, Rebecca Schwab, Melanie Hilpert, Marcelo Desimone, Rainer Schwacke, Ulf-Ingo Flügge, Karin Schumacher, Wolf B. Frommer |
| Summary: | Complementation of a yeast acr1 mutant carrying a deletion of the succinate/fumarate carrier gene enabled functional identification of a mitochondrial succinate translocator from Arabidopsis thaliana (AtmSFC1). Thus complementation of yeast mutants is applicable also for identification and characterization of organellar transporters. Reverse transcription polymerase chain reaction and promoter-GUS fusion showed expression of AtmSFC1 in 2 day old dark grown seedlings, which declined in cotyledons during further development, consistent with a role in export of fumarate for gluconeogenesis during lipid mobilization at early germination of Arabidopsis seeds. In mature plants, expression was found in developing and germinating pollen, suggesting a role in ethanolic fermentation. |
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| Item Description: | First published: 12 December 2002 Gesehen am 07.10.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/S0014-5793(02)03782-1 |