Cover Image

Structural characterization of an Arf dimer interface: molecular mechanism of Arf-dependent membrane scission

Dimerization of the small GTPase Arf is prerequisite for the scission of COPI-coated transport vesicles. Here, we quantify the monomer/dimer equilibrium of Arf within the membrane and show that after membrane scission, Arf dimers are restricted to donor membranes. By hydrogen exchange mass spectrome...

Full description

Saved in:
Bibliographic Details
Main Authors: Diestelkötter-Bachert, Petra (Author) , Beck, Rainer (Author) , Reckmann, Ingeborg (Author) , Hellwig, Andrea (Author) , Garcia-Saez, Ana (Author) , Zelman-Hopf, Monika (Author) , Hanke, Anton (Author) , Nunes Alves, Ariane (Author) , Wade, Rebecca C. (Author) , Mayer, Matthias P. (Author) , Wieland, Felix T. (Author)
Format: Article (Journal)
Language:English
Published: 2020
In: FEBS letters
Year: 2020, Volume: 594, Issue: 14, Pages: 2240-2253
ISSN:1873-3468
DOI:10.1002/1873-3468.13808
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1002/1873-3468.13808
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13808
Get full text
Author Notes:Petra Diestelkoetter-Bachert, Rainer Beck, Inge Reckmann, Andrea Hellwig, Ana Garcia-Saez, Monika Zelman-Hopf, Anton Hanke, Ariane Nunes Alves, Rebecca C. Wade, Matthias P. Mayer, and Felix Wieland
Description
Summary:Dimerization of the small GTPase Arf is prerequisite for the scission of COPI-coated transport vesicles. Here, we quantify the monomer/dimer equilibrium of Arf within the membrane and show that after membrane scission, Arf dimers are restricted to donor membranes. By hydrogen exchange mass spectrometry, we define the interface of activated dimeric Arf within its switch II region. Single amino acid exchanges in this region reduce the propensity of Arf to dimerize. We suggest a mechanism of membrane scission by which the dimeric form of Arf is segregated to the donor membrane. Our data are consistent with the previously reported absence of dimerized Arf in COPI vesicles and could explain the presence of one single scar-like noncoated region in each COPI vesicle.
Item Description:First published: 11 May 2020
Gesehen am 10.11.2021
Physical Description:Online Resource
ISSN:1873-3468
DOI:10.1002/1873-3468.13808

Similar Items