Palmitoylated proteins in plasmodium falciparum-infected erythrocytes: investigation with click chemistry and metabolic labeling
The examination of the complex cell biology of the human malaria parasite Plasmodium falciparum usually relies on the time-consuming generation of transgenic parasites. Here, metabolic labeling and click chemistry are employed as a fast transfection-independent method for the microscopic examination...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2020
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| In: |
Bioessays
Year: 2020, Volume: 42, Issue: 6, Pages: 1-10 |
| ISSN: | 1521-1878 |
| DOI: | 10.1002/bies.201900145 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/bies.201900145 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/bies.201900145 
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| Author Notes: | Nicole Kilian, Yongdeng Zhang, Lauren LaMonica, Giles Hooker, Derek Toomre, Choukri Ben Mamoun, and Andreas M. Ernst |
| Summary: | The examination of the complex cell biology of the human malaria parasite Plasmodium falciparum usually relies on the time-consuming generation of transgenic parasites. Here, metabolic labeling and click chemistry are employed as a fast transfection-independent method for the microscopic examination of protein S-palmitoylation, an important post-translational modification during the asexual intraerythrocytic replication of P. falciparum. Applying various microscopy approaches such as confocal, single-molecule switching, and electron microscopy, differences in the extent of labeling within the different asexual developmental stages of P. falciparum and the host erythrocytes over time are observed. |
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| Item Description: | First published: 28 April 2020 Gesehen am 16.11.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1521-1878 |
| DOI: | 10.1002/bies.201900145 |