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Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera

Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi-quantitative high-content microscopy-based assay for detection of three major classes (IgG, IgA, and IgM) of SARS-Co...

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Main Authors: Pape, Constantin (Author) , Remme, Roman (Author) , Wolny, Adrian (Author) , Olberg, Sylvia (Author) , Wolf, Steffen (Author) , Cerrone, Lorenzo (Author) , Cortese, Mirko (Author) , Klaus, Severina (Author) , Lucic, Bojana (Author) , Ullrich, Stephanie (Author) , Anders-Ößwein, Maria (Author) , Wolf, Stefanie (Author) , Cerikan, Berati (Author) , Neufeldt, Christopher (Author) , Ganter, Markus (Author) , Schnitzler, Paul (Author) , Merle, Uta (Author) , Lusic, Marina (Author) , Boulant, Steeve (Author) , Stanifer, Megan (Author) , Bartenschlager, Ralf (Author) , Hamprecht, Fred (Author) , Kreshuk, Anna (Author) , Tischer, Christian (Author) , Kräusslich, Hans-Georg (Author) , Müller, Barbara (Author) , Laketa, Vibor (Author)
Format: Article (Journal)
Language:English
Published: 2021
In: Bioessays
Year: 2021, Volume: 43, Issue: 3, Pages: 1-15
ISSN:1521-1878
DOI:10.1002/bies.202000257
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1002/bies.202000257
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/bies.202000257
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Author Notes:Constantin Pape, Roman Remme, Adrian Wolny, Sylvia Olberg, Steffen Wolf, Lorenzo Cerrone, Mirko Cortese, Severina Klaus, Bojana Lucic, Stephanie Ullrich, Maria Anders-Össwein, Stefanie Wolf, Berati Cerikan, Christopher J. Neufeldt, Markus Ganter, Paul Schnitzler, Uta Merle, Marina Lusic, Steeve Boulant, Megan Stanifer, Ralf Bartenschlager, Fred A. Hamprecht, Anna Kreshuk, Christian Tischer, Hans-Georg Kräusslich, Barbara Müller, Vibor Laketa
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Summary:Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi-quantitative high-content microscopy-based assay for detection of three major classes (IgG, IgA, and IgM) of SARS-CoV-2 specific antibodies in human samples. The possibility to detect antibodies against the entire viral proteome together with a robust semi-automated image analysis workflow resulted in specific, sensitive and unbiased assay that complements the portfolio of SARS-CoV-2 serological assays. Sensitive, specific and quantitative serological assays are urgently needed for a better understanding of humoral immune response against the virus as a basis for developing public health strategies to control viral spread. The procedure described here has been used for clinical studies and provides a general framework for the application of quantitative high-throughput microscopy to rapidly develop serological assays for emerging virus infections.
Item Description:First published: 30 December 2020
Gesehen am 02.12.2021
Physical Description:Online Resource
ISSN:1521-1878
DOI:10.1002/bies.202000257

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