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Phagocytosis by stroma confounds coculture studies

Signals provided by the microenvironment can modify and circumvent pathway activities that are therapeutically targeted by drugs. Bone marrow stromal cell coculture models are frequently used to study the influence of the bone marrow niche on ex vivo drug response. Here, we show that mesenchymal str...

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Main Authors: Herbst, Sophie (Author) , Stolarczyk, Marta (Author) , Becirovic, Tina (Author) , Czernilofsky, Felix (Author) , Liu, Yi (Author) , Kolb, Carolin (Author) , Knoll, Mareike (Author) , Herling, Marco (Author) , Müller-Tidow, Carsten (Author) , Dietrich, Sascha (Author)
Format: Article (Journal)
Language:English
Published: 30 August 2021
In: iScience
Year: 2021, Volume: 24, Issue: 9, Pages: 1-15
ISSN:2589-0042
DOI:10.1016/j.isci.2021.103062
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.isci.2021.103062
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S2589004221010300
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Author Notes:Sophie A. Herbst, Marta Stolarczyk, Tina Becirovic, Felix Czernilofsky, Yi Liu, Carolin Kolb, Mareike Knoll, Marco Herling, Carsten Müller-Tidow, and Sascha Dietrich
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Summary:Signals provided by the microenvironment can modify and circumvent pathway activities that are therapeutically targeted by drugs. Bone marrow stromal cell coculture models are frequently used to study the influence of the bone marrow niche on ex vivo drug response. Here, we show that mesenchymal stromal cells from selected donors and NKTert, a stromal cell line, which is commonly used for coculture studies with primary leukemia cells, extensively phagocytose apoptotic cells. This could lead to misinterpretation of results, especially if viability readouts of the target cells (e.g. leukemic cells) in such coculture models are based on the relative proportions of dead and alive cells. Future coculture studies which aim to investigate the impact of bone marrow stromal cells on drug response should take into account that stromal cells have the capacity to phagocytose apoptotic cells.
Item Description:Gesehen am 02.12.2021
Physical Description:Online Resource
ISSN:2589-0042
DOI:10.1016/j.isci.2021.103062

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