Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGF
Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
08 Oct 2013
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| In: |
Radiology and oncology
Year: 2013, Volume: 47, Issue: 4, Pages: 330-337 |
| ISSN: | 1581-3207 |
| DOI: | 10.2478/raon-2013-0063 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.2478/raon-2013-0063 Verlag, lizenzpflichtig, Volltext: https://sciendo.com/article/10.2478/raon-2013-0063 
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| Author Notes: | Neza Podergajs, Narve Brekka, Bernhard Radlwimmer, Christel Herold-Mende, Krishna M. Talasila, Katja Tiemann, Uros Rajcevic, Tamara T. Lah, Rolf Bjerkvig, Hrvoje Miletic |
| Summary: | Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without EGF, as the presence of EGF induced rapid loss of EGFR amplification. However, patient biopsies are usually taken into culture before their genomic profiles are defined. Thus, an important question remains whether GBM cells without EGFR amplification also can be cultured in stem cell medium without EGF. |
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| Item Description: | Gesehen am 03.12.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1581-3207 |
| DOI: | 10.2478/raon-2013-0063 |