Supported membranes meet flat fluidics: monitoring dynamic cell adhesion on pump-free microfluidics chips functionalized with supported membranes displaying mannose domains
In this paper we demonstrate the combination of supported membranes and so-called flat microfluidics, which enables one to manipulate liquids on flat chip surfaces via “inverse piezoelectric effect”. Here, an alternating external electric field applied to the inter-digital transducers excites a sur...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
22 February 2013
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| In: |
Materials
Year: 2013, Volume: 6, Issue: 2, Pages: 669-681 |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma6020669 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/ma6020669 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1996-1944/6/2/669 
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| Author Notes: | Jochen Oelke, Thomas Kaindl, Andreea Pasc, Zeno Guttenberg, Achim Wixforth and Motomu Tanaka |
| Summary: | In this paper we demonstrate the combination of supported membranes and so-called flat microfluidics, which enables one to manipulate liquids on flat chip surfaces via “inverse piezoelectric effect”. Here, an alternating external electric field applied to the inter-digital transducers excites a surface acoustic wave on a piezoelectric substrate. Employing lithographic patterning of self-assembled monolayers of alkoxysilanes, we successfully confine a free-standing, hemi-cylindrical channel with the volume of merely 7 µL . The experimentally determined maximum flow velocity scales linearly with the acoustic power, suggesting that our current setup can drive liquids at the speed of up to 7 cm/s (corresponding to a shear rate of 280 s−1) without applying high pressures using a fluidic pump. After the establishment of the functionalization of fluidic chip surfaces with supported membranes, we deposited asymmetric supported membranes displaying well-defined mannose domains and monitored the dynamic adhesion of E. Coli HB101 expressing mannose-binding receptors. Despite of the further technical optimization required for the quantitative analysis, the obtained results demonstrate that the combination of supported membranes and flat fluidics opens a large potential to investigate dynamic adhesion of cells on biofunctional membrane surfaces with the minimum amount of samples, without any fluidic pump. |
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| Item Description: | Gesehen am 06.12.2021 |
| Physical Description: | Online Resource |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma6020669 |