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Identification and separation of normal hematopoietic stem cells and leukemia stem cells from patients with acute myeloid leukemia

Mounting evidences indicate that leukemic cells in patients with acute myeloid leukemia (AML) are derived from leukemia stem cells (LSC). In analogy to normal hematopoietic stem cells (HSC), LSC remain mostly dormant and are hence resistant to conventional chemotherapy. Residual, physiological HSC e...

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Main Authors: Hoang, Thanh Van (Author) , Hoffmann, Isabel (Author) , Borowski, Karina (Author) , Zepeda-Moreno, Abraham (Author) , Ran, Dan (Author) , Buß, Eike Christian (Author) , Wuchter, Patrick (Author) , Eckstein, Volker (Author) , Ho, Anthony Dick (Author)
Format: Chapter/Article
Language:English
Published: 13 June 2013
In: Stem cell niche
Year: 2013, Pages: 217-230
DOI:10.1007/978-1-62703-508-8_19
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/978-1-62703-508-8_19
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Author Notes:Van T. Hoang, Isabel Hoffmann, Karina Borowski, Abraham Zepeda-Moreno, Dan Ran, Eike C. Buss, Patrick Wuchter, Volker Eckstein, Anthony D. Ho
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Summary:Mounting evidences indicate that leukemic cells in patients with acute myeloid leukemia (AML) are derived from leukemia stem cells (LSC). In analogy to normal hematopoietic stem cells (HSC), LSC remain mostly dormant and are hence resistant to conventional chemotherapy. Residual, physiological HSC exist alongside with LSC, with heterogeneous dominance of LSC over HSC in individual patients. We have devised a flow cytometric method for the identification and separation of these two stem cell populations based on surface antigen markers such as CD34, CD38, lineage aberrant markers, and aldehyde dehydrogenase (ALDH) enzyme activity.
Item Description:Gesehen am 14.12.2021
Physical Description:Online Resource
ISBN:9781627035088
DOI:10.1007/978-1-62703-508-8_19

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