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RNAi suppressor P19 can be broadly exploited for enhanced adenovirus replication and microRNA knockdown experiments

RNA interference (RNAi) is a key regulator of various biological systems including viral infection. Within a virus life cycle gene products can be modulated by the RNA interference (RNAi) pathway which can crucially impact productive virus replication. Herein we explored the RNA interference suppres...

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Bibliographic Details
Main Authors: Rauschhuber, Christina Theresa (Author) , Mueck-Haeusl, Martin (Author) , Zhang, Wenli (Author) , Nettelbeck, Dirk M. (Author) , Ehrhardt, Anja (Author)
Format: Article (Journal)
Language:English
Published: 4 March 2013
In: Scientific reports
Year: 2013, Volume: 3, Issue: 1, Pages: 1-7
ISSN:2045-2322
DOI:10.1038/srep01363
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/srep01363
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/srep01363
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Author Notes:Christina Rauschhuber, Martin Mueck-Haeusl, Wenli Zhang, Dirk M. Nettelbeck & Anja Ehrhardt
Description
Summary:RNA interference (RNAi) is a key regulator of various biological systems including viral infection. Within a virus life cycle gene products can be modulated by the RNA interference (RNAi) pathway which can crucially impact productive virus replication. Herein we explored the RNA interference suppressor protein P19 derived from a plant virus and we found that P19 enhanced adenovirus replication up to 100-fold. Critical factors responsible for this observation were overexpression of adenovirus encoded genes on mRNA and protein levels. To investigate the impact of this phenomenon on recombinant viruses, we exploited its feasibility for therapeutic and genomic applications. We found that P19 significantly increased recombinant adenovirus yields enabling up-scaling for preclinical and clinical studies. Moreover, adenoviruses possessed significantly higher oncolytic activity by expression of P19. Finally, we show that introducing a p19 expression cassette into high-capacity adenovirus provides a strategy to analyze RNAi knockdown in a tissue-specific manner.
Item Description:Gesehen am 16.12.2021
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/srep01363

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