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Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms

Algorithms that account for and overcome the intrinsic pixel-dependent readout noise from scientific complementary metal-oxide semiconductor (sCMOS) cameras prevent localization artifacts and avoid substantial compromises in localization uncertainty in single-molecule imaging. When combined with mul...

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Main Authors: Huang, Fang (Author) , Hartwich, Tobias Max Philipp (Author) , Rivera-Molina, Felix E. (Author) , Lin, Yu (Author) , Duim, Whitney C. (Author) , Long, Jane J. (Author) , Uchil, Pradeep D. (Author) , Myers, Jordan R. (Author) , Baird, Michelle A. (Author) , Mothes, Walther (Author) , Davidson, Michael W. (Author) , Toomre, Derek (Author) , Bewersdorf, Jörg (Author)
Format: Article (Journal)
Language:English
Published: 26 May 2013
In: Nature methods
Year: 2013, Volume: 10, Issue: 7, Pages: 653-658
ISSN:1548-7105
DOI:10.1038/nmeth.2488
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nmeth.2488
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nmeth.2488
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Author Notes:Fang Huang, Tobias MP Hartwich, Felix E Rivera-Molina, Yu Lin, Whitney C Duim, Jane J Long, Pradeep D Uchil, Jordan R Myers, Michelle A Baird, Walther Mothes, Michael W Davidson, Derek Toomre & Joerg Bewersdorf
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Summary:Algorithms that account for and overcome the intrinsic pixel-dependent readout noise from scientific complementary metal-oxide semiconductor (sCMOS) cameras prevent localization artifacts and avoid substantial compromises in localization uncertainty in single-molecule imaging. When combined with multi-emitter fitting, sCMOS cameras allow video-rate single-molecule switching nanoscopy.
Item Description:Gesehen am 05.01.2022
Physical Description:Online Resource
ISSN:1548-7105
DOI:10.1038/nmeth.2488

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