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Dnmt2-dependent methylomes lack defined DNA methylation patterns

Several organisms have retained methyltransferase 2 (Dnmt2) as their only candidate DNA methyltransferase gene. However, information about Dnmt2-dependent methylation patterns has been limited to a few isolated loci and the results have been discussed controversially. In addition, recent studies hav...

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Bibliographic Details
Main Authors: Raddatz, Günter (Author) , Guzzardo, Paloma M. (Author) , Olova, Nelly (Author) , Fantappié, Marcelo Rosado (Author) , Rampp, Markus (Author) , Schaefer, Matthias R. (Author) , Reik, Wolf (Author) , Hannon, Gregory J. (Author) , Lyko, Frank (Author)
Format: Article (Journal)
Language:English
Published: 2013
In: Proceedings of the National Academy of Sciences of the United States of America
Year: 2013, Volume: 110, Issue: 21, Pages: 8627-8631
ISSN:1091-6490
DOI:10.1073/pnas.1306723110
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.1306723110
Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/content/110/21/8627
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Author Notes:Günter Raddatz, Paloma M. Guzzardo, Nelly Olova, Marcelo Rosado Fantappié, Markus Rampp, Matthias Schaefer, Wolf Reik, Gregory J. Hannon, and Frank Lyko
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Summary:Several organisms have retained methyltransferase 2 (Dnmt2) as their only candidate DNA methyltransferase gene. However, information about Dnmt2-dependent methylation patterns has been limited to a few isolated loci and the results have been discussed controversially. In addition, recent studies have shown that Dnmt2 functions as a tRNA methyltransferase, which raised the possibility that Dnmt2-only genomes might be unmethylated. We have now used whole-genome bisulfite sequencing to analyze the methylomes of Dnmt2-only organisms at single-base resolution. Our results show that the genomes of Schistosoma mansoni and Drosophila melanogaster lack detectable DNA methylation patterns. Residual unconverted cytosine residues shared many attributes with bisulfite deamination artifacts and were observed at comparable levels in Dnmt2-deficient flies. Furthermore, genetically modified Dnmt2-only mouse embryonic stem cells lost the DNA methylation patterns found in wild-type cells. Our results thus uncover fundamental differences among animal methylomes and suggest that DNA methylation is dispensable for a considerable number of eukaryotic organisms.
Item Description:Gesehen am 11.01.2022
Physical Description:Online Resource
ISSN:1091-6490
DOI:10.1073/pnas.1306723110

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