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A combination of granulocyte-colony-stimulating factor (G-CSF) and plerixafor mobilizes more primitive peripheral blood progenitor cells than G-CSF alone: results of a European phase II study

Background aims - Previous studies in xenograft models have shown that human peripheral blood progenitor cells (PBPC) mobilized with the CXCR4 antagonist plerixafor (AMD3100) have a higher bone marrow (BM) reconstitution potential than granulocyte-colony-stimulating factor (G-CSF)-mobilized PBPC. -...

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Main Authors: Frühauf, Stefan (Author) , Veldwijk, Marlon Romano (Author) , Seeger, Timon (Author) , Schubert, Mario (Author) , Maier-Laufs, Stephanie (Author) , Topaly, Julian (Author) , Wuchter, Patrick (Author) , Dillmann, Falk (Author) , Eckstein, Volker (Author) , Wenz, Frederik (Author) , Goldschmidt, Hartmut (Author) , Ho, Anthony Dick (Author) , Calandra, Gary (Author)
Format: Article (Journal)
Language:English
Published: [2009]
In: Cytotherapy
Year: 2009, Volume: 11, Issue: 8, Pages: 992-1001
ISSN:1477-2566
DOI:10.3109/14653240903121245
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3109/14653240903121245
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1465324909703470
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Author Notes:Stefan Fruehauf, Marlon Romano Veldwijk, Timon Seeger, Mario Schubert, Stephanie Laufs, Julian Topaly, Patrick Wuchter, Falk Dillmann, Volker Eckstein, Frederik Wenz, Hartmut Goldschmidt, Anthony Dick Ho and Gary Calandra
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Summary:Background aims - Previous studies in xenograft models have shown that human peripheral blood progenitor cells (PBPC) mobilized with the CXCR4 antagonist plerixafor (AMD3100) have a higher bone marrow (BM) reconstitution potential than granulocyte-colony-stimulating factor (G-CSF)-mobilized PBPC. - Methods - PBPC obtained during G-CSF-supported mobilization before and after a supplementary administration of AMD3100 from patients with multiple myeloma and non-Hodgkin's lymphoma (n=15; phase II study) were investigated for co-expression of primitive and lineage-associated markers, their proliferative activity in vitro and repopulation potential after clinical transplantation. - Results - A significant increase in primitive CD34+ CD38− cells was observed in intraindividual comparisons of all patients after administration of G-CSF+AMD3100 (peripheral blood: median 8-fold, range 2,4-fold - 39-fold) compared with G-CSF alone. Using a long-term culture-initiating cell assay, this increase was confirmed. After transplantation of G-CSF+AMD3100-mobilized PBPC, the time to leukocyte reconstitution >1×103/μL and platelet reconstitution >2×104/μL was 14 (10-19 days) and 13 days (10-15 days), respectively. A complete and stable hematologic reconstitution (platelets >1.5×105/μL) was observed in 91% of all patients within 35 days. - Conclusions - An additional application of AMD3100 to a standard G-CSF mobilization regimen leads to a significant increase in primitive PBPC with high repopulation capacity.
Item Description:Gesehen am 12.01.2022
Physical Description:Online Resource
ISSN:1477-2566
DOI:10.3109/14653240903121245

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