Development of high-throughput multiplex serology to detect serum antibodies against Coxiella burnetii
The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investiga...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
17 November 2021
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| In: |
Microorganisms
Year: 2021, Volume: 9, Issue: 11, Pages: 1-11 |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9112373 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/microorganisms9112373 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2076-2607/9/11/2373 
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| Author Notes: | Rima Jeske, Larissa Dangel, Leander Sauerbrey, Dimitrios Frangoulidis, Lauren R. Teras, Silke F. Fischer and Tim Waterboer |
| Summary: | The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine C. burnetii proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect C. burnetii infections. Com1, GroEL, and DnaK achieved specificities of 93%, 69%, and 77% and sensitivities of 64%, 72%, and 47%, respectively. Double positivity to Com1 and GroEL led to a combined specificity of 90% and a sensitivity of 71%. In a subgroup of seropositives with an increased risk for chronic Q fever, the double positivity to these markers reached a specificity of 90% and a sensitivity of 86%. Multiplex serology enables the detection of antibodies against C. burnetii and appears well-suited to investigate associations between C. burnetii infections and the clinical manifestations in large-scale studies. |
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| Item Description: | Gesehen am 19.01.2022 |
| Physical Description: | Online Resource |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9112373 |