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Soluble bone marrow stroma factors improve the efficiency of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells

Hematopoietic stem cells (HSCs) are a potential target for the retrovirus-mediated transfer of chemotherapeutic drug resistance genes. For integration of the proviral DNA in the HSC genome cell division is required. In the bone marrow (BM) hematopoiesis occurs in the vicinity of stroma cells. Solubl...

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Bibliographic Details
Main Authors: Schiedlmeier, Bernhard (Author) , Buß, Eike Christian (Author) , Veldwijk, Marlon Romano (Author) , Zeller, W. Jens (Author) , Frühauf, Stefan (Author)
Format: Article (Journal)
Language:English
Published: June 10, 1999
In: Human gene therapy
Year: 1999, Volume: 10, Issue: 9, Pages: 1443-1452
ISSN:1557-7422
DOI:10.1089/10430349950017789
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1089/10430349950017789
Verlag, lizenzpflichtig, Volltext: https://www.liebertpub.com/doi/10.1089/10430349950017789
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Author Notes:B. Schiedlmeier, E.C. Buss, M.R. Veldwijk, W.J. Zeller, and S. Fruehauf
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Summary:Hematopoietic stem cells (HSCs) are a potential target for the retrovirus-mediated transfer of chemotherapeutic drug resistance genes. For integration of the proviral DNA in the HSC genome cell division is required. In the bone marrow (BM) hematopoiesis occurs in the vicinity of stroma cells. Soluble stroma components were shown to play a permissive role for the proliferation of lineage-committed and primitive hematopoietic progenitors in conjunction with cytokines. We investigated the effect of stroma-conditioned medium (SCM) of the FBMD1 cell line on the gene transfer rate of the human multidrug resistance 1 (MDR1) gene contained in the retroviral SF-MDR vector into human mobilized peripheral blood progenitor cells (PBPCs) from tumor patients (n = 14) during transwell transduction in the presence of the recombinant fibronectin fragment CH-296.
Item Description:Elektronische Reproduktion der Druck-Ausgabe
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Physical Description:Online Resource
ISSN:1557-7422
DOI:10.1089/10430349950017789

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