Contact-mediated quenching for RNA imaging in bacteria with a fluorophore-binding aptamer
Bind and shine: Sulforhodamine B was converted into a very efficient fluorescence turn-on probe exclusively by contact quenching. The fluorescence of the probe increases more than 100-fold upon binding to a sulforhodamine-binding RNA aptamer (SRB-2). The probe and the SRB-2 aptamer were used to moni...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
October 16, 2013
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| In: |
Angewandte Chemie. International edition
Year: 2013, Volume: 52, Issue: 50, Pages: 13401-13404 |
| ISSN: | 1521-3773 |
| DOI: | 10.1002/anie.201306622 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/anie.201306622 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201306622 
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| Author Notes: | Murat Sunbul and Andres Jäschke |
| Summary: | Bind and shine: Sulforhodamine B was converted into a very efficient fluorescence turn-on probe exclusively by contact quenching. The fluorescence of the probe increases more than 100-fold upon binding to a sulforhodamine-binding RNA aptamer (SRB-2). The probe and the SRB-2 aptamer were used to monitor transcriptions in real-time and to image RNA in live bacteria. |
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| Item Description: | Gesehen am 27.01.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1521-3773 |
| DOI: | 10.1002/anie.201306622 |