Parallelizable microfluidic platform to model and assess in vitro cellular barriers: technology and application to study the interaction of 3d tumor spheroids with cellular barriers
Endothelial and epithelial cellular barriers play a vital role in the selective transport of solutes and other molecules. The properties and function of these barriers are often affected in case of inflammation and disease. Modelling cellular barriers in vitro can greatly facilitate studies of infla...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
3 September 2021
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| In: |
Biosensors
Year: 2021, Volume: 11, Issue: 9, Pages: 1-16 |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11090314 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/bios11090314 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2079-6374/11/9/314 
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| Author Notes: | Arya Lekshmi Nair, Lena Mesch, Ingo Schulz, Holger Becker, Julia Raible, Heiko Kiessling, Simon Werner, Ulrich Rothbauer, Christian Schmees, Marius Busche, Sebastian Trennheuser, Gert Fricker and Martin Stelzle |
| Summary: | Endothelial and epithelial cellular barriers play a vital role in the selective transport of solutes and other molecules. The properties and function of these barriers are often affected in case of inflammation and disease. Modelling cellular barriers in vitro can greatly facilitate studies of inflammation, disease mechanisms and progression, and in addition, can be exploited for drug screening and discovery. Here, we report on a parallelizable microfluidic platform in a multiwell plate format with ten independent cell culture chambers to support the modelling of cellular barriers co-cultured with 3D tumor spheroids. The microfluidic platform was fabricated by microinjection molding. Electrodes integrated into the chip in combination with a FT-impedance measurement system enabled transepithelial/transendothelial electrical resistance (TEER) measurements to rapidly assess real-time barrier tightness. The fluidic layout supports the tubeless and parallelized operation of up to ten distinct cultures under continuous unidirectional flow/perfusion. The capabilities of the system were demonstrated with a co-culture of 3D tumor spheroids and cellular barriers showing the growth and interaction of HT29 spheroids with a cellular barrier of MDCK cells. |
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| Item Description: | Gesehen am 01.02.2022 |
| Physical Description: | Online Resource |
| ISSN: | 2079-6374 |
| DOI: | 10.3390/bios11090314 |