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Analysis of 5′-NAD capping of mRNAs in dormant spores of Bacillus subtilis

Spores of Gram-positive bacteria contain 10s-1000s of different mRNAs. However, Bacillus subtilis spores contain only ∼ 50 mRNAs at > 1 molecule/spore, almost all transcribed only in the developing spore and encoding spore proteins. However, some spore mRNAs could be stabilized to ensure they are...

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Main Authors: Craft, Levi Darien (Author) , Korza, George (Author) , Zhang, Yaqing (Author) , Frindert, Jens (Author) , Jäschke, Andres (Author) , Caimano, Melissa J. (Author) , Setlow, Peter (Author)
Format: Article (Journal)
Language:English
Published: 21 August 2020
In: FEMS microbiology letters
Year: 2020, Volume: 367, Issue: 17, Pages: 1-8
ISSN:1574-6968
DOI:10.1093/femsle/fnaa143
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1093/femsle/fnaa143
Verlag, kostenfrei, Volltext: https://academic.oup.com/femsle/article/doi/10.1093/femsle/fnaa143/5895323
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Author Notes:D. Levi Craft, George Korza, Yaqing Zhang, Jens Frindert, Andres Jäschke, Melissa J. Caimano and Peter Setlow
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Summary:Spores of Gram-positive bacteria contain 10s-1000s of different mRNAs. However, Bacillus subtilis spores contain only ∼ 50 mRNAs at > 1 molecule/spore, almost all transcribed only in the developing spore and encoding spore proteins. However, some spore mRNAs could be stabilized to ensure they are intact in dormant spores, perhaps to direct synthesis of proteins essential for spores’ conversion to a growing cell in germinated spore outgrowth. Recent work shows that some growing B. subtilis cell mRNAs contain a 5 -NAD cap. Since this cap may stabilize mRNA in vivo, its presence on spore mRNAs would suggest that maintaining some intact spore mRNAs is important, perhaps because they have a translational role in outgrowth. However, significant levels of only a few abundant spore mRNAs had a 5 -NAD cap, and these were not the most stable spore mRNAs and had likely been fragmented. Even higher levels of 5 -NAD-capping were found on a few low abundance spore mRNAs, but these mRNAs were present in only small percentages of spores, and had again been fragmented. The new data are thus consistent with spore mRNAs serving only as a reservoir of ribonucleotides in outgrowth.
Item Description:Gesehen am 03.02.2022
Physical Description:Online Resource
ISSN:1574-6968
DOI:10.1093/femsle/fnaa143

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