Polarization optical-histochemical characterization and supramolecular structure of carbohydrate fibrils

Topo-optical staining reactions were used to investigate the structures of bacterial cellulose, insect chitosan and alginic acid from brown algae. Polysaccharide complexes, glycosaminoglycans and sulfate groups were presented and demonstrated selectively. Chitosan and alginic acid are structurally s...

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Main Authors: Csóka, Levente (Author) , Appel, Thomas R. (Author) , Eitner, Annett (Author) , Jirikowski, Gustav (Author) , Makovitzky, József (Author)
Format: Article (Journal)
Language:English
Published: 2013
In: Acta histochemica
Year: 2013, Volume: 115, Issue: 1, Pages: 22-31
ISSN:1618-0372
DOI:10.1016/j.acthis.2012.03.003
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.acthis.2012.03.003
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0065128112000487
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Author Notes:Levente Csoka, Thomas R. Appel, Annett Eitner, Gustav Jirikowski, Josef Makovitzky
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Summary:Topo-optical staining reactions were used to investigate the structures of bacterial cellulose, insect chitosan and alginic acid from brown algae. Polysaccharide complexes, glycosaminoglycans and sulfate groups were presented and demonstrated selectively. Chitosan and alginic acid are structurally similar to glycosaminoglycans (GAGs), which are constituents of human amyloid fibrils. The staining sequences shown can be used as reliable methods for histochemistry with light and polarization microscopy. They will help to clarify the complex protein-polysaccharide structure of amyloid fibrils.
Item Description:Gesehen am 16.02.2022
Available online 10 April 2012
Physical Description:Online Resource
ISSN:1618-0372
DOI:10.1016/j.acthis.2012.03.003