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Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases

Early steps of cancer initiation and metastasis, while critical for understanding disease mechanisms, are difficult to visualize and study. Here, we describe an approach to study the processes of initiation, progression, and metastasis of prostate cancer (PC) in a genetically engineered RapidCaP mou...

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Main Authors: Taranda, Julian (Author) , Mathew, Grinu (Author) , Watrud, Kaitlin (Author) , El-Amine, Nour (Author) , Lee, Matthew F. (Author) , Elowsky, Corey (Author) , Bludova, Anastasiia (Author) , Escobar Avelar, Sintia (Author) , Nowak, Dawid G. (Author) , Wee, Tse-Luen (Author) , Wilkinson, John E. (Author) , Trotman, Lloyd C. (Author) , Osten, Pavel (Author)
Format: Article (Journal)
Language:English
Published: November 16, 2021
In: Cell reports
Year: 2021, Volume: 37, Issue: 7, Pages: 1-13
ISSN:2211-1247
DOI:10.1016/j.celrep.2021.110027
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2021.110027
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S2211124721015096
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Author Notes:Julian Taranda, Grinu Mathew, Kaitlin Watrud, Nour El-Amine, Matthew F. Lee, Corey Elowsky, Anastasiia Bludova, Sintia Escobar Avelar, Dawid G. Nowak, Tse-Luen Wee, John E. Wilkinson, Lloyd C. Trotman, and Pavel Osten
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Summary:Early steps of cancer initiation and metastasis, while critical for understanding disease mechanisms, are difficult to visualize and study. Here, we describe an approach to study the processes of initiation, progression, and metastasis of prostate cancer (PC) in a genetically engineered RapidCaP mouse model, which combines whole-organ imaging by serial two-photon tomography (STPT) and post hoc thick-section immunofluorescent (IF) analysis. STPT enables the detection of single tumor-initiating cells within the entire prostate, and consequent IF analysis reveals a transition from normal to transformed epithelial tissue and cell escape from the tumor focus. STPT imaging of the liver and brain reveal the distribution of multiple metastatic foci in the liver and an early-stage metastatic cell invasion in the brain. This imaging and data analysis pipeline can be readily applied to other mouse models of cancer, offering a highly versatile whole-organ platform to study in situ mechanisms of cancer initiation and progression.
Item Description:Gesehen am 03.03.2022
Physical Description:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2021.110027

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