The impact of culture conditions on early follicle recruitment and growth from human ovarian cortex biopsies in vitro
Objective - To investigate the effects of a dynamic fluidic culture system on early in vitro folliculogenesis in standardized ovarian cortex biopsies. - Design - Cortical small strips were cultured for 6 days in a conventional static or in a dynamic fluidic culture system. - Setting - University-aff...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
April 28, 2013
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| In: |
Fertility and sterility
Year: 2013, Volume: 100, Issue: 2, Pages: 483-491.e5 |
| ISSN: | 1556-5653 |
| DOI: | 10.1016/j.fertnstert.2013.03.046 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.fertnstert.2013.03.046 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0015028213004627 
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| Author Notes: | Jana Liebenthron, M.Sc., Maria Köster, D.V.M., Christina Drengner, Jochen Reinsberg, Ph.D., Hans van der Ven, M.D., and Markus Montag, Ph.D. |
| Summary: | Objective - To investigate the effects of a dynamic fluidic culture system on early in vitro folliculogenesis in standardized ovarian cortex biopsies. - Design - Cortical small strips were cultured for 6 days in a conventional static or in a dynamic fluidic culture system. - Setting - University-affiliated laboratory with an associated cryobank facility. - Patient(s) - Ovarian cortex from postpuberal female cancer patients (26.1 ± 1.3 y) who opted for cryopreservation of their tissue for fertility protection before gonadotoxic cancer therapy. With informed consent of the Institutional Ethics Committee, part of the tissue was available for patient-related research studies. - Intervention(s) - None. - Main Outcome Measure(s) - The viability and proliferative capacity of the cortex biopsies were evaluated by chemiluminescent microparticle immunoassay for detection of in vitro produced E2 and P in the supernate, by viable follicle counting via calcein staining, by histologic analyses, and by total RNA preparation and reverse transcription for real-time polymerase chain reaction of selected early folliculogenesis genes. - Result(s) - The data support the notion that early follicle development can be better achieved in vitro in a dynamic fluidic culture system. The findings are based on the presence of more viable follicles, higher expression levels of early folliculogenesis genes KIT-L, INHB, and GDF9, and the absence of premature luteinization of follicles. - Conclusion(s) - This study provides evidence that dynamic fluidic culture is a promising approach for investigating early follicular recruitment and growth in cortical biopsies. It may serve as a first step in a multistep culture system to design a complex in vitro system for complete folliculogenesis. |
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| Item Description: | Gesehen am 02.03.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1556-5653 |
| DOI: | 10.1016/j.fertnstert.2013.03.046 |