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Cotranslational N-degron masking by acetylation promotes proteome stability in plants

N-terminal protein acetylation (NTA) is a prevalent protein modification essential for viability in animals and plants. The dominant executor of NTA is the ribosome tethered Nα-acetyltransferase A (NatA) complex. However, the impact of NatA on protein fate is still enigmatic. Here, we demonstrate th...

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Main Authors: Linster, Eric (Author) , Forero Ruiz, Francy Liliana (Author) , Miklánková, Pavlína (Author) , Ruppert, Thomas (Author) , Mueller, Johannes (Author) , Armbruster, Laura (Author) , Gong, Xiaodi (Author) , Serino, Giovanna (Author) , Mann, Matthias (Author) , Hell, Rüdiger (Author) , Wirtz, Markus (Author)
Format: Article (Journal)
Language:English
Published: 10 February 2022
In: Nature Communications
Year: 2022, Volume: 13, Pages: 1-12
ISSN:2041-1723
DOI:10.1038/s41467-022-28414-5
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-022-28414-5
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-022-28414-5
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Author Notes:Eric Linster, Francy L. Forero Ruiz, Pavlina Miklankova, Thomas Ruppert, Johannes Mueller, Laura Armbruster, Xiaodi Gong, Giovanna Serino, Matthias Mann, Rüdiger Hell & Markus Wirtz
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Summary:N-terminal protein acetylation (NTA) is a prevalent protein modification essential for viability in animals and plants. The dominant executor of NTA is the ribosome tethered Nα-acetyltransferase A (NatA) complex. However, the impact of NatA on protein fate is still enigmatic. Here, we demonstrate that depletion of NatA activity leads to a 4-fold increase in global protein turnover via the ubiquitin-proteasome system in Arabidopsis. Surprisingly, a concomitant increase in translation, actioned via enhanced Target-of-Rapamycin activity, is also observed, implying that defective NTA triggers feedback mechanisms to maintain steady-state protein abundance. Quantitative analysis of the proteome, the translatome, and the ubiquitome reveals that NatA substrates account for the bulk of this enhanced turnover. A targeted analysis of NatA substrate stability uncovers that NTA absence triggers protein destabilization via a previously undescribed and widely conserved nonAc/N-degron in plants. Hence, the imprinting of the proteome with acetylation marks is essential for coordinating proteome stability.
Item Description:Gesehen am 30.03.2022
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-022-28414-5

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