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Common T-cell-receptor motifs and features in patients with cytomegalovirus (CMV)-seronegative end-stage renal disease receiving a peptide caccination against CMV

After solid-organ transplantation, reactivation of the cytomegalovirus (CMV) is often observed in seronegative patients and associated with a high risk of disease and mortality. CMV-specific T cells can prevent CMV reactivation. In a phase 1 trial, CMV-seronegative patients with end-stage renal dise...

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Main Authors: Bunse, Lukas (Author) , Sommerer, Claudia (Author) , Tan, Chin Leng (Author) , Korell, Felix (Author) , Schmitt, Anita (Author) , Hückelhoven-Krauss, Angela (Author) , Neuber, Brigitte (Author) , Mertens, Thomas (Author) , Platten, Michael (Author) , Green, Edward W. (Author) , Zeier, Martin (Author) , Schmitt, Michael (Author)
Format: Article (Journal)
Language:English
Published: 18 January 2022
In: International journal of molecular sciences
Year: 2022, Volume: 23, Issue: 3, Pages: 1-12
ISSN:1422-0067
DOI:10.3390/ijms23031029
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/ijms23031029
Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1422-0067/23/3/1029
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Author Notes:Lukas Bunse, Claudia Sommerer, Chin Leng Tan, Felix Korell, Anita Schmitt, Angela Hückelhoven-Krauss, Brigitte Neuber, Thomas Mertens, Michael Platten, Edward W. Green, Martin Zeier and Michael Schmitt
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Summary:After solid-organ transplantation, reactivation of the cytomegalovirus (CMV) is often observed in seronegative patients and associated with a high risk of disease and mortality. CMV-specific T cells can prevent CMV reactivation. In a phase 1 trial, CMV-seronegative patients with end-stage renal disease listed for kidney transplantation were subjected to CMV phosphoprotein 65 (CMVpp65) peptide vaccination and further investigated for T-cell responses. To this end, CMV-specific CD8+ T cells were characterized by bulk T-cell-receptor (TCR) repertoire sequencing and combined single-cell RNA and TCR sequencing. In patients mounting an immune response to the vaccine, a common SYE(N)E TCR motif known to bind CMVpp65 was detected. CMV-peptide-vaccination-responder patients had TCR features distinct from those of non-responders. In a non-responder patient, a monoclonal inflammatory T-cell response was detected upon CMV reactivation. The identification of vaccine-induced CMV-reactive TCRs motifs might facilitate the development of cellular therapies for patients wait-listed for kidney transplantation.
Item Description:Gesehen am 30.03.2022
Physical Description:Online Resource
ISSN:1422-0067
DOI:10.3390/ijms23031029

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