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Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium

The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profil...

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Hauptverfasser: Vickovic, Sanja (VerfasserIn) , Schapiro, Denis (VerfasserIn) , Carlberg, Konstantin (VerfasserIn) , Lötstedt, Britta (VerfasserIn) , Larsson, Ludvig (VerfasserIn) , Hildebrandt, Franziska (VerfasserIn) , Korotkova, Marina (VerfasserIn) , Hensvold, Aase H. (VerfasserIn) , Catrina, Anca I. (VerfasserIn) , Sorger, Peter K. (VerfasserIn) , Malmström, Vivianne (VerfasserIn) , Regev, Aviv (VerfasserIn) , Ståhl, Patrik L. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 11 February 2022
In: Communications biology
Year: 2022, Jahrgang: 5, Pages: 1-11
ISSN:2399-3642
DOI:10.1038/s42003-022-03050-3
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s42003-022-03050-3
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s42003-022-03050-3
Volltext
Verfasserangaben:Sanja Vickovic, Denis Schapiro, Konstantin Carlberg, Britta Lötstedt, Ludvig Larsson, Franziska Hildebrandt, Marina Korotkova, Aase H. Hensvold, Anca I. Catrina, Peter K. Sorger, Vivianne Malmström, Aviv Regev & Patrik L. Ståhl
Beschreibung
Zusammenfassung:The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics, where tissue-resident RNA is spatially labeled in situ with barcodes in a transcriptome-wide fashion, to study local tissue interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-Seq data coupled to cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium. Combining morphological features and high-throughput spatially resolved transcriptomics may be able to provide higher statistical power and more insights into monitoring disease severity and treatment-specific responses in seropositive and seronegative rheumatoid arthritis.
Beschreibung:Gesehen am 22.03.2022
Beschreibung:Online Resource
ISSN:2399-3642
DOI:10.1038/s42003-022-03050-3

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