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Three-dimensional spatial transcriptomics uncovers cell type localizations in the human rheumatoid arthritis synovium

The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profil...

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Main Authors: Vickovic, Sanja (Author) , Schapiro, Denis (Author) , Carlberg, Konstantin (Author) , Lötstedt, Britta (Author) , Larsson, Ludvig (Author) , Hildebrandt, Franziska (Author) , Korotkova, Marina (Author) , Hensvold, Aase H. (Author) , Catrina, Anca I. (Author) , Sorger, Peter K. (Author) , Malmström, Vivianne (Author) , Regev, Aviv (Author) , Ståhl, Patrik L. (Author)
Format: Article (Journal)
Language:English
Published: 11 February 2022
In: Communications biology
Year: 2022, Volume: 5, Pages: 1-11
ISSN:2399-3642
DOI:10.1038/s42003-022-03050-3
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s42003-022-03050-3
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s42003-022-03050-3
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Author Notes:Sanja Vickovic, Denis Schapiro, Konstantin Carlberg, Britta Lötstedt, Ludvig Larsson, Franziska Hildebrandt, Marina Korotkova, Aase H. Hensvold, Anca I. Catrina, Peter K. Sorger, Vivianne Malmström, Aviv Regev & Patrik L. Ståhl
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Summary:The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics, where tissue-resident RNA is spatially labeled in situ with barcodes in a transcriptome-wide fashion, to study local tissue interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-Seq data coupled to cell type-specific localization patterns at and around organized structures of infiltrating leukocyte cells in the synovium. Combining morphological features and high-throughput spatially resolved transcriptomics may be able to provide higher statistical power and more insights into monitoring disease severity and treatment-specific responses in seropositive and seronegative rheumatoid arthritis.
Item Description:Gesehen am 22.03.2022
Physical Description:Online Resource
ISSN:2399-3642
DOI:10.1038/s42003-022-03050-3

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