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Investigation of tumor hypoxia using a two-enzyme system for in vitro generation of oxygen deficiency

Oxygen deficiency in tumor tissue is associated with a malign phenotype, characterized by high invasiveness, increased metastatic potential and poor prognosis. Hypoxia chambers are the established standard model for in vitro studies on tumor hypoxia. An enzymatic hypoxia system (GOX/CAT) based on th...

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Hauptverfasser: Askoxylakis, Vasileios (VerfasserIn) , Millonig, Gunda (VerfasserIn) , Wirkner, Ute (VerfasserIn) , Schwager, Christian (VerfasserIn) , Rana, Shoaib (VerfasserIn) , Altmann, Annette (VerfasserIn) , Haberkorn, Uwe (VerfasserIn) , Debus, Jürgen (VerfasserIn) , Mueller, Sebastian (VerfasserIn) , Huber, Peter E. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 10 April 2011
In: Radiation oncology
Year: 2011, Jahrgang: 6, Pages: 1-12
ISSN:1748-717X
DOI:10.1186/1748-717X-6-35
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1186/1748-717X-6-35
Volltext
Verfasserangaben:Vasileios Askoxylakis, Gunda Millonig, Ute Wirkner, Christian Schwager, Shoaib Rana, Annette Altmann, Uwe Haberkorn, Jürgen Debus, Sebastian Mueller and Peter E Huber
Beschreibung
Zusammenfassung:Oxygen deficiency in tumor tissue is associated with a malign phenotype, characterized by high invasiveness, increased metastatic potential and poor prognosis. Hypoxia chambers are the established standard model for in vitro studies on tumor hypoxia. An enzymatic hypoxia system (GOX/CAT) based on the use of glucose oxidase (GOX) and catalase (CAT) that allows induction of stable hypoxia for in vitro approaches more rapidly and with less operating expense has been introduced recently. Aim of this work is to compare the enzymatic system with the established technique of hypoxia chamber in respect of gene expression, glucose metabolism and radioresistance, prior to its application for in vitro investigation of oxygen deficiency.
Beschreibung:Gesehen am 23.03.2022
Beschreibung:Online Resource
ISSN:1748-717X
DOI:10.1186/1748-717X-6-35

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